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Vegetable Research at the North Willamette Research and Extension Center, 1997-­1998

CONTENTS

Introduction
Nitrogen Management in Sweet Corn
Effect of Winter Cover Crops on Vegetable Crop Yield and Leaching of Nitrate
Impact of Cover Crop and Previous Vegetable Crop on Mold Incidence in Snapbean
Cultivar, N Rate, and Row Spacing Affect Yield of Edamame

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AUTHOR

Dr. Delbert D. Hemphill, Jr., Professor of Horticulture, has conducted research on vegetable crops culture and management since 1976 at Oregon State University's North Willamette Research and Extension Center, 15210 NE Miley Rd., Aurora, OR 97002-9543.


COOPERATORS

Dr. John Hart is Extension Soil Scientist and Professor, Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331

Dr. Richard Dick is Professor of Soil Science, Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331

Dr. John Selker is Associate Professor, Department of Bioresources Engineering, Oregon State University, Corvallis, OR 97331

Dr. Carol Miles is a Washington State University Extension Agent, 360 NW North St., MS:AESOL, Chehalis, WA 98532

Dr. Mary Powelson is Professor, Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331

Dr. Neil Christensen is Professor, Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331

Introduction to the Report

We have conducted a full-time program of vegetable crop research at the North Willamette Research and Extension Center (formerly the North Willamette Experiment Station) since 1976. The Center, a branch of both Oregon State University's Agricultural Experiment Station and its Extension Service, is just north of Aurora, a historic farming community 20 miles south of Portland, Oregon. The land is provided by Clackamas County, with facilities owned and maintained by OSU. The Center serves the vegetable, small fruit, wine grape, and nursery crops industries and is located in an area noted for the diversity of its agriculture. Our vegetable research emphasizes the needs of both the processed vegetable and the fresh market vegetable growers in the Willamette Valley. We also conduct research on home garden and small-farm intensive vegetable culture.

Many of the research projects reported here involved cooperation with Experiment Station and Extension Service colleagues at OSU and at Washington State University. Their contributions are gratefully acknowledged. The financial support of the Oregon Processed Vegetable Commission was essential to completing these projects and is greatly appreciated.

This report is the eleventh in a series of biennial reports initiated in 1979.

DISCLAIMER: The use of trade names does not constitute an endorsement by the Oregon State University Agricultural Experiment Station. Always check pesticide labels for currently registered uses.


Nitrogen Management in Sweet Corn

Cooperators: Dr. John Hart, Dept. of Crop and Soil Science, Oregon State University and Dr. Carol Miles, Extension Service, Washington State University

Introduction

Vegetable growers in the Willamette Valley use high rates of N fertilizers, often exceeding 250 to 300 pounds actual N/acre per season. While growers believe that these rates are necessary to achieve maximum yields and quality, a considerable portion of the applied fertilizer is not taken up by the crop (Hemphill, 1997). This excess has raised concerns that the remaining N may be contributing to nitrate pollution of ground or surface waters. We feel that our research has contributed significantly to an understanding of the yield response of vegetables to N fertilizer and the critical stages for N uptake, particularly as related to residual mineral N after harvest. Both research at the North Willamette Research and Extension Center (NWREC) and a three-year survey of residual mineral N in grower fields indicates that residual N tends to be greatest following sweet corn. For the crops that have been the major focus of our research (sweet corn, broccoli, cauliflower), we feel that more work on rates of fertilizer application, timing, placement,and N source will not lead to significant changes in the way we fertilize these crops.

We have recently focused on the most important remaining questions and have moved toward solutions to the problem of high levels of residual applied N following vegetable crops. We have attempted to develop methods to predict crop response to sidedressed N and to determine how much sidedressed N is needed, particularly in sweet corn. Research on silage corn grown with high inputs of manures indicates that a pre-sidedress soil nitrate test (PSNT) may be useful in determining the amount of additional N needed (Marx, 1995). In 1995, we started to extend testing of this method to sweet corn, basing our initial efforts on the results of research in New Jersey (Heckman and Prostak, 1992). Our preliminary evaluations of the PSNT in 1995 and 1996 (Hemphill, 1997) convinced us that the potential exists to reduce sidedress N applications to sweet corn significantly in certain circumstances, resulting in reduced cost to growers and reduction in leachable residual nitrate. The results reported here continued this effort and resulted in a modification of the PSNT, which appears applicable to sweet corn production on typical Willamette Valley soils.

Methods, NWREC Experiment, 1997

'Jubilee' sweet corn was seeded on 15 May on 30-inch row spacing. The plots had previously been fallow for two years. Nitrogen rates of 0, 50, 100, 150, and 200 pounds/acre were applied at planting to establish five different levels of early-season soil nitrate (Table 2). Soil samples for PSNT were taken on 7 July and sidedress N rates of 0, 30, 60, 90, and 150 pounds/A were applied to the appropriate plots on 10 July. All N was applied as urea. Table 1 contains the complete list of treatments. One treatment (13) received 50 pounds N/acre at planting, followed by 25-pound applications on 10, 18, and 29 July, and 12 August, for a total N application of 150 pounds/acre. SPAD-502 chlorophyll meter (Minolta Co., Ltd., Japan) readings were taken on intact leaves on 11, 18, and 29 July, and 12 and 21 August. Harvest was on 20 August. Plots were sampled to 1-foot depth for residual soil nitrate on 21 August.

Methods, NWREC Experiment, 1998

'Jubilee' sweet corn was seeded on 9 June on 30-inch rows. The plots had previously been fallow for two years. Nitrogen rates of 0, 40, 80, 120, and 180 pounds/acre were applied at planting to establish five different levels of early-season soil nitrate (Table 7). Soil samples for PSNT were taken on 21 July. Sidedress N rates of 0, 20, 60, 80, 100, 140, and 200 pounds/A were applied to the appropriate plots on 22 and 29 July, and 14 August, to complete 11 different combinations of N rate, form of N applied, and timing of N application (Table 2). All N was applied as urea except for applications of Greenfeed (TM) 27-0-0 liquid controlled release N solution to corn foliage on Treatments 15 and 16 (Table 3). The Greenfeed material contains 7.1% urea plus 19.4% urea derivatives (polymethylene urea, monomethylol urea, methylene diurea) which break down relatively slowly. SPAD meter readings were taken on 10 intact leaves/plot at approximately weekly intervals starting on 16 July and continuing at approximately weekly intervals through harvest. Harvest was on 14 September. Plots were sampled for residual soil nitrate on 18 September. A second variety, GH 1703 (Rogers Sandoz), was also seeded on 9 June and treated with only 5 rates of N (Table 3). The purpose was to compare the performance of the SPAD meter over two varieties.

Methods, On-farm Trial of the Pre-sidedress Soil Nitrate Test (PSNT), 1997 and 1998

As in 1996 (Hemphill, 1997), the PSNT was evaluated in 1997 and 1998 at nine grower locations in which plots of approximately 1 acre were compared to the grower practice in the remainder of the field for ear yield, stalk nitrate-N at harvest, and soil nitrate-N at harvest. The assumption of the PSNT is that soil can be tested at about the 1-foot stage of corn growth (about 5 weeks after planting) and that sidedress fertilizer application can be reduced when soil nitrate content exceeds a critical level. The soil was sampled to a depth of 12 inches and analyzed for nitrate-N content. Sufficient corn was harvested from the plot area to fill a truck. The harvested area was measured, the contents of the truck weighed, and the yield calculated. A similar procedure was followed to provide a matching sample from an adjacent portion of the field in which the standard grower practice was followed. At harvest, 12-inch segments of corn stalks from just above the brace roots were cut, dried, ground, and analyzed for nitrate-N concentration. A soil sample was taken at the same time and also analyzed for nitrate-N content.

In 1997, growers limited pre-plant and at-planting N application to 50 pounds/acre. Treatments were the grower's standard sidedress N rate and one-half this rate. Three of the sites were replicated. Sites considered responsive to N were those where yield from the half-rate of sidedress N was less than 98% of the yield from the full rate of sidedress N. A relative yield of 98% was chosen as the yield below which the cost of additional N fertilizer would be recovered in additional yield. For the calculation, we assumed a price of $85/ton for corn, a 10 ton/acre yield, and a cost for N of $0.32/lb.

In 1998, our goal was to have 175 pounds available N/acre provided from a combination of the soil and sidedress N application. The soil samples were analyzed for nitrate-N and an approximation of the amount of nitrate-N in the soil (pounds/acre) was made using the conversion parts/million multiplied by 3.6 = pounds/acre in the surface foot of soil. Sufficient sidedress N was applied to provide a total of 175 pounds N/acre. The fertilizer N was applied by the growers. A relative yield level of 97.5% was chosen based on the current prices for urea fertilizer ($195/ton), the value of sweet corn at $85/ton, and fertilizer application cost of $5/acre. At this relative yield, a yield increase of 0.25 tons/acre would just offset the cost of an additional 75 pounds N/acre. Thus, a relative yield level of 97.5% is a reasonable measure of success of the PSNT prediction for needed N with 1998 prices.

Results, NWREC, 1997

Our past trials indicated that the critical level of PSNT is about 25 ppm; that is, a positive yield-response to additional N is not to be expected when the soil nitrate concentration is at this level 4 to 6 weeks after planting. However, in this experiment, application of 100 pounds N/acre at planting resulted in a PSNT of 47 ppm (Table 2) but this level of N was not adequate for maximum yield (Table 4). This is probably because achieving this level of available N as a result of fertilizer application does not indicate a continuing reservoir of available N coming from mineralization of organic matter. In contrast, in the grower trials, much lower levels of N were usually applied at planting, resulting in a better estimate of nitrate-N availability from the soil.

The highest yield was obtained with 200 pounds N/acre, with all the N applied at planting. Yields tended to decline at higher N rates. Past trials have indicated greater yield with split N application. In this trial a breakdown of fertilizer banding equipment prevented banding N below the seedline. A broadcast application of the initial 50 lb N/acre (Treatment 14) may not have provided sufficient N during early growth. In general, our experiments have indicated that, while it is advisable to delay the application of the bulk of crop N needs until after rainfall has tapered off, it is critical to have adequate N present at all times. This can be seen clearly in the case of Treatment 13 (Table 4), for which yield was much lower than other treatments with similar N rates (Trts. 4, 5, 6, 12). Apparently, delaying the application of much of the N until relatively late in crop development meant N deficiencies at critical earlier stages of growth.

Sweet corn yield is plotted vs. total N rate in Figure 1. Most yields clustered between 8 and 9 tons/acre, so a smooth curve cannot be fitted to the data. However, three points stand out: the high yields at 200 lb N/acre and the low yield of 7.6 tons/acre for Trt. 13. SPAD readings also tend to cluster (Table 5 and Figure 2) but the relationship between SPAD and N applied can be seen in Figure 2. SPAD reading at harvest increased linearly with rate of N applied up to about 180 pounds N/acre. After this, the SPAD reading tends to plateau and not be responsive to more N. There is no good explanation for the one obvious outlier at 200 lb applied N. A similar relationship existed for earlier SPAD readings.

Figs. 1 and 2

Figure 3 demonstrates how SPAD readings tend to vary during the season. Typically, the readings start low, increase, and then gradually decline toward harvest. The middle curve in the figure is atypical. This is the curve for the 150 lb treatment (Trt. 13) in which the N was applied in six separate applications of 25 or 50 lb/acre. Dribbling on the fertilizer in this fashion resulted in very steady SPAD readings but, as seen above, yield suffered. Figure 4 indicates that there is not a simple relationship between SPAD at harvest and yield. Points are clustered between SPAD readings of 37 and 44. This is because of the great variation in this experiment among different N rates at planting and at harvest. The timing of the N application as well as the total amount applied affects the SPAD reading. For SPAD to be an effective tool in sweet corn fertilization, we will need to generate more data of the type in Figure 3 and standardize the method and timing of N application.

Figs. 3 and 4

Both soil nitrate and ammonium concentrations were elevated at time of harvest, but only for high rates of applied N (Table 6). The high levels of residual N at 150 pounds applied N/acre were likely due to half the total N being applied to this treatment (#13) after the normal sidedress time. Almost 80 pounds nitrate-N/acre were left behind in the surface foot of soil by the corn crop fertilized with 200 pounds N/acre.

Results, NWREC, 1998

As noted above, preliminary trials in 1995 and 1996 indicated that the critical level of PSNT is about 25 ppm. In this experiment, application of 120 pounds N/acre at planting resulted in a PSNT of 29.6 ppm (Table 7). A treatment which provided a total N application of 120 lb/acre (80 at planting, 40 sidedress at mid-season) produced maximum yield (Tables 8 and 9), indicating that a PSNT level of 25­30 ppm might have produced maximum yield, but this was not tested directly.

The highest yield of both 'Jubilee' and 'GH 1703' was obtained with 120 pounds N/acre, with 80 pounds/acre applied at planting and the remainder 6 weeks later. Yields tended to decline at higher N rates. Past trials have indicated greater yield with split N application, but with maximum yield at 180-200 lb/acre. One explanation for the highest yields at a lower-than-expected N rate may be seen in Table 7. Available N in the soil before planting was unusually high, nearly 50 pounds N/acre as nitrate and 15 as ammonium. If this amount of available N already present in the soil is added to the 120 pounds/acre applied N, then the highest yield was obtained at 185 pounds available N/acre, very much consistent with previous trials. This serves to reinforce the importance of the PSNT, as high concentrations of available N are occasionally present in Willamette Valley soils, even after a high-rainfall winter like that of 1997­1998.

Averaged over all N rates, 'GH 1703' outyielded 'Jubilee' by 0.9 tons/acre (Table 8). However, the difference in yield was greater at the suboptimal rates of N (Table 9). Mean ear weight and tipfill responded less to increasing N rate for 'GH 1703' than for 'Jubilee'. The two varieties also had a strikingly different appearance in the field. Leaves of 'GH 1703' were darker green and thicker, and the color remained dark green until harvest even at the zero rate of N. If confirmed in future testing, the ability of a variety to produce acceptable yields at lower rates of applied N may be of great help in avoiding leaching of nitrate and in reducing fertilizer costs.

Treatments 11 through 14 were added to compare the performance of 'Jubilee' at 180 pounds/acre applied N, but with varying rates applied at planting versus mid-season and later sidedressings. Yields of these treatments are seen in rows 4­7 of Table 10. None of these treatments produced a higher yield or quality than the standard method of 40 pounds N/acre at planting and 140 pounds/acre sidedressed 6 weeks after planting (Treatment 7, 10.1 tons/acre, line 7 in Table 9). While applying relatively small amounts of N at several times during the season could affect residual soil N or N leaching, it is clear from this and previous work at NWREC that there is no yield advantage to delaying N applications past mid-season. However, note from Table 11 that treatments 11, 12, and 13 (lines 6, 7, and 8) resulted in lower residual soil N than did applying all the sidedress N at one time (line 4).

In treatments 15 and 16, we compared corn yield with all or a portion of the sidedress N applied as Greenfeed 27-0-0 controlled-release N at 60 and 120 pounds N/acre, respectively, with the corresponding treatments in which all sidedress was applied as urea on 22 July (compare Table 10, lines 2 and 3 with Table 9, lines 3 and 5). The use of Greenfeed 27-0-0 did not provide a yield advantage compared to the use of urea alone as N source.

SPAD readings started off higher than last year, reflecting the higher level of nitrate in the soil at planting. At time of sidedress N application, SPAD readings did not vary with application of either 0 or 40 lb N/acre at planting (Table 12). Differences among total N rates were not evident until two weeks later. SPAD readings for treatments receiving suboptimal N started to decline 3 to 4 weeks before harvest. The thicker, darker green leaves of 'GH 1703' gave consistently higher SPAD readings than did 'Jubilee', indicating that separate calibration curves will be needed for each variety. As in 1997, applying small amounts of sidedress N four times during the growing season tended to keep SPAD readings more level over time (Table 13) but, as noted above, did not result in improved yield.

As in 1997, both soil nitrate and ammonium concentrations were elevated at time of harvest but only for high rates of applied N of 180 pounds/acre or more (Table 11). The residual nitrate-N level of about 40 pounds/acre for the optimal N rate of 120 pounds/acre is probably acceptable and certainly is no higher than at planting. However, at 180 and 240 pounds N/acre, residual nitrate-N varied from 60 to 160 pounds/acre, depending on N rate and timing of application. Residual nitrate levels were generally higher in 1998 than in 1997 for all but the lowest rates of applied N. Residual ammonium levels did not vary significantly with amount of N applied.

Results, On-farm PSNT Trial, 1997

PSNT values ranged from 8.8 to 38.8 ppm nitrate-N, similar to those found the previous two years (Table 14). The conclusion from 1996 was that 100 pounds sidedress N/acre was sufficient for at least 98% relative yield when the PSNT value was 18 ppm or more. Our approach in 1997 was slightly different as the sidedress N rates varied by grower rather than being set at 100 vs. 150 pounds/acre. In addition to this variation in experimental procedure, the analysis of the data was modified.

Relative yield was plotted vs. the sum of soil nitrate-N (as determined by PSNT) and sidedressed fertilizer N (Fig. 5). The horizontal line in the Cate-Nelson type graph represents 98 percent relative yield. The goal of the Cate- Nelson technique is to manipulate the vertical and horizontal lines such that the greatest number of points is located in the upper right (I) and lower left (IV) quadrants of the graph. Points in these two quadrants are viewed as "correct" predictions. The lower left quadrant (IV) represents situations in which the PSNT would have called for additional N and prediction was correct. The points in the upper right quadrant (I) represent situations in which the test indicated that available N was adequate for 98 percent relative yield and the prediction was correct.

Points falling in the upper left and lower right quadrants are considered "incorrect" predictions. The upper left quadrant (III) represents situations in which the PSNT called for additional N, but the relative yield indicates that it was not needed. These incorrect predictions are of low risk to the grower as they call for an additional fertilizer application that the grower would likely make anyway unless that grower typically uses rates that are considerably less than most growers now apply. Yield would not be sacrificed and expenditures would be the same save for the cost of the PSNT itself. However, these situations are potentially the most damaging to the environment because excess N at the end of the growing season will be leached by the heavy winter rainfall typical of western Oregon.

Points falling in the lower right quadrant (II) are situations where the plots did not achieve a relative yield of 98 percent when the PSNT indicated that N was adequate. These sites were on sandy or gravelly soils in which the reservoir of mineralizable soil N was very low.

Yield data from both 1996 and 1997 is presented in Figure 6. The graph reveals that 98 percent or greater relative yield was attained when the sum of the PSNT and sidedress N was greater than 175 pounds/acre. If the sum of PSNT and sidedress N is less than 150 pounds/acre the relative yield is less than 98 percent. Between 150 and 175 pounds/acre, the relative yield is sometimes less than 98 percent and sometimes greater than 98 percent.

Figs. 5 and 6.

Assuming that the sum of PSNT plus sidedress N must be 175 pounds/acre to achieve the desired relative yield of 98 percent, the amount of sidedress N to apply can be calculated from the formula:

Sidedress N (pounds/acre) = 175 pounds/acre - [PSNT (ppm) x 3.6] (1)

Another method to provide sidedress N rates is to group PSNT results into three categories as shown in Table 15.

Stalk nitrate concentrations at harvest (Table 14) did not provide an adequate assessment of yield or N treatment. The critical value of 2700 ppm that we proposed in 1996 (Hemphill, 1997) seems very low for 1997 data. In addition, a sufficient nitrate-N concentration of 10,000 ppm in stalks at harvest was found in New Jersey (Heckman and Prostak, 1992). Our current data set shows that high stalk nitrate-N content, probably above 8,000 ppm, indicated adequate N supply, but that stalk concentrations below 8,000 ppm did not necessarily indicate insufficient N supply.

Results, On-farm PSNT Trial, 1998

The 1998 trial was a test of the formula (1) proposed above. Yields ranged from a low of 7.2 tons/acre at site G, which had an infestation of barnyard grass, to a high of nearly 14 tons/acre at site B (Table 16). To compare yield from all sites, relative yield was calculated by dividing the yield of the PSNT plot by the yield from the sample of the rest of the field and multiplying by 100 percent. For example, if yield from the field was 10.0 tons/acre and that from the PSNT plot was 9.75 tons/acre, the relative yield is 97.5 percent.

Relative yield was lowest (92.3 percent) at site E and highest (103.8 percent) at site B. Relative yield is ranked against the sum of sidedress N fertilizer and the soil nitrate-N level determined by the PSNT (Table 17). The lowest amount of N, 133 pounds/acre, was entirely soil N. No N fertilizer was sidedressed at this site. The plot yield was almost 96 percent of the yield from the rest of the field which had a sidedress N application of 160 lb/acre. Four of the plots received less than 175 pounds N/acre as a combination of soil N and sidedress N. Of these four sites, only one (A) produced a relative yield greater than 97.5 percent and that site had a combined soil and applied N level of 170 pounds/acre.

Of the five sites with more than 175 pounds N/acre from soil and sidedress, two produced relative yields less than 97.5 percent. Site H was a sandy soil; at harvest soil and stalk nitrate-N content at site H were lower for the plot area than for the rest of the field. Soil and stalk nitrate-N content at harvest were both low for site D. The stalk NO3-N concentration was lower in the plot area than in the field (Table 16).

Relative yields for the last three years were plotted versus the sum of sidedress and soil N (Figure 7). The vertical line on the right of Figure 7 represents 175 pounds/acre; the left vertical line marks 135 pounds N/acre. This line creates a third category of data points where additional N produces additional yield about half the time i.e., between 135 and 175 pounds N/acre there are five points above, and five points below, the 97.5 percent relative yield line. Another way to express the test outcome is "yes, maybe, no." If sidedress plus soil N is below 135 pounds/acre, then, "yes" ‹a yield increase from additional N is highly likely. If the soil plus sidedress N is above 175 pounds/acre, then, "no" ‹increased yield with additional N in highly unlikely except on sandy or gravelly soils. If the N level is between 135 and 175, then, "maybe" ‹a yield increase with more N is difficult to predict. For fields in the "maybe" category, the SPAD meter may be useful in monitoring plant N status.

Fig. 7.

For the last three years, nitrate-N content of the soil at time of PSNT ranged from 30 to 160 pounds/acre in the surface foot. This range of available N shows the need for a test that enables growers to adjust sidedress N application rates on a site-specific basis. Our modification of the PSNT allows many growers to make this adjustment. Of the 30 locations reported here, a detrimental economic consequence of using the modified PSNT occurred in only two locations.

Literature Cited

Heckman, J.R. and D. Prostak. 1992. Presidedress soil nitrate test (PSNT) recommendations for sweet corn. Rutgers Coop. Ext. and N.J. Agric. Expt. Sta. FS 760.

Hemphill, D.D., Jr. 1997. Vegetable research at the North Willamette Research and Extension Center, 1995­1996. Oregon Agric. Expt. Sta. Spec. Rep. No. 975.

Marx, E. 1995. Evaluation of soil and plant analyses as components of a nitrogen monitoring program for silage corn. M.S. Thesis, Oregon State Univ., Corvallis.

Table 1. List of N rates (lb/acre), PSNT trial, NWREC, 1997.
Trt.   Total N rate   At planting   Sidedress, 7/10          
1           0             0           None
2          80            50             30
3         110            50             60
4         140            50             90
5         130           100             30
6         160           100             60
7         190           100             90
8         180           150             30
9         210           150             60
10        240           150             90
11        200           200              0
12        150           150              0
13        150            50           4x25
14        200            50            150                  


Table 2.  Effect of N at planting on soil nitrate levels
and leaf SPAD readings at time of PSNT testing, NWREC, 1997.
N at planting, lb/acre  Soil nitrate, ppm, 7/7   SPAD, 7/11     
  0                              3.7                37.3
 50                             20.3                42.0
100                             47.0                43.8
150                             56.4                44.9
200                             71.3                44.9
      LSD (0.05)                35.5                 4.2    
  


Table 3. List of treatments in PSNT sweet corn experiment,
NWREC, 1998.                                                 
Trt.  Variety   Total N    N at           Sidedress N     
                applied  planting     7/22    7/29    8/12 
                ----------------lb/acre-------------------
1     Jubilee        0       0          0       0       0
2     GH 1703        0       0          0       0       0
3     Jubilee       60      40         20       0       0
4     GH 1703       60      40         20       0       0
5     Jubilee      120      40         80       0       0
6     GH 1703      120      40         80       0       0
7     Jubilee      180      40        140       0       0
8     GH 1703      180      40        140       0       0
9     Jubilee      240      40        200       0       0
10    GH 1703      240      40        200       0       0
11    Jubilee      180      40         60      40      40
12    Jubilee      180      80         40      40      20
13    Jubilee      180     120         20      20      20
14    Jubilee      180     180          0       0       0
15    Jubilee       60      40         10z      0      10z
16    Jubilee      120      40         40y     20z     20z
__________________________________________________________
zApplied as Greenfeed 27-0-0 controlled release N solution.
yApplied as one-half urea, one-half Greenfeed 27-0-0.


Table 4. Yield of sweet corn, PSNT plots, NWREC, 1997.      
Trt.   Total N     Yield     Ear length   Ear wt.  Tipfill
      (lb/acre) (tons/acre)   (inches)     g/ear               
1          0       3.4          8.4         249      2.3z
2         80       8.0          9.1         321      3.1
3        110       8.5          9.7         320      3.1
5        130       8.1          9.6         316      2.9
4        140       8.6          9.8         317      3.0
12       150       8.8          9.7         324      3.0
13       150       7.6          9.5         296      2.8
6        160       8.2          9.8         330      3.1
8        180       8.7          9.7         319      3.1
7        190       8.5          9.5         332      3.2
11       200      10.1          9.5         321      3.2
14       200       9.5          9.4         283      2.9
9        210       8.9          9.6         349      3.1
10       240       8.9          9.2         314      2.9
     LSD (0.05)    1.6          0.5          28      0.6    
zFive-point scale with 5 = perfect fill, 1 = 2 inches
or more unfilled kernels.


Table 5. Trends in SPAD readings as affected by total N application, NWREC, 1997.  
Trt.  Total N, lb/acre   SPAD, 7/18    SPAD, 7/29    SPAD, 8/12    SPAD, 8/21      
1               0           38.5          29.5          22.9          19.5        
2              80           48.7          50.8          40.9          34.7
3             110           47.3          47.7          39.7          38.0
5             130           49.8          52.4          43.9          38.9
4             140           48.1          48.8          43.3          40.3
12            150           50.8          50.0          41.8          37.7
6             160           50.3          51.8          44.4          42.5
8             180           50.2          52.5          46.3          44.5
7             190           52.0          49.8          45.2          43.4
11, 14        200           49.7          51.5          44.7          41.3
9             210           51.0          53.2          42.6          43.4
10            240           50.4          53.2          45.0          42.3
13            150z          44.0 (75)     43.4 (100)    43.0 (125)    42.7 (150)  
        LSD (0.05)           2.8           5.2           5.9           4.7        
zTotal at end of season.  Actual amounts applied before each SPAD measurement
are in parentheses after the SPAD mean.  


Table 6. Effect of total N rate on residual soil N levels in the surface
foot of soil, NWREC, 1997.                                              
Total N, lb/acre   Residual nitrate, ppm     Residual ammonium, ppm          
  0                           0.9                       4.6
 80                           2.7                       6.0
110                           2.9                       5.2
140                           4.0                       6.3
150z                         14.9                      14.3
200                          19.9                      13.3
             LSD (0.05)       5.2                        NS                
zThis treatment (#13) had a large proportion of the total N applied 
relatively late in the season.


Table 7. Effect of N applied at planting on soil nitrate and
ammonium levels at time of sidedress N application, NWREC, 1998. 
N rate, lb/acre    Soil nitrate-N, ppm    Soil ammonium-N, ppm  
  0                       13.1                    3.8
 40                       22.3                    4.1
 80                       23.8                    5.1
120                       29.6                    6.4
180                       31.1                    9.5
        Significance       ***                    ***           
***Significant at 0.1% level.


Table 8. Main effects of rate of applied N and cultivar on yield of 
sweet corn, NWREC, 1998.                                             
                Yield     No. of     Mean ear   Tipfillz    Ear length
              tons/acre  ears/plot    wt. (g)                 inches   
N rate, lb/A
  0              7.3        55         210        2.2         7.7
 60              9.2        58         251        2.6         8.3
120             10.9        62         277        2.9         8.6
180             10.4        59         275        2.8         8.5
240             10.4        59         275        2.7         8.6
  Significance   ***         *         ***         *           **

Cultivar
Jubilee          9.2        59         244        2.7         8.4
GH 1703         10.1        58         272        2.5         8.2
  Significance   **         NS         ***         NS          NS    
z5-point scale with 5 = perfect fill, 1 = 2 or more inches unfilled.
***,**,*,NSSignificant at p=0.1, 1, and 5% levels, and non-significant,
respectively.


Table 9. Interaction of rate of applied N and cultivar on yield of sweet 
corn, NWREC, 1988.                                                         
N rate      Cultivar     Yield     No. of    Mean ear  Tipfillz  Ear length
lb/acre                tons/acre  ears/plot   wt. (g)              inches  
  0         Jubilee       6.3        55         179      1.8        7.6
            GH 1703       8.4        54         241      2.5        7.8
 60         Jubilee       8.7        58         233      2.6        8.4
            GH 1703       9.7        57         268      2.5        8.2
120         Jubilee      10.7        63         265      3.1        8.8
            GH 1703      11.2        60         290      2.7        8.3
180         Jubilee      10.1        59         266      3.2        8.9
            GH 1703      10.8        59         284      2.3        8.0
240         Jubilee      10.4        59         276      3.0        8.6
            GH 1703      10.4        59         275      2.5        8.6
     LSD (0.05)           0.6         5          30                 0.1    
z5-point scale with 5 = perfect fill, 1 = 2 or more inches unfilled.


Table 10. Effects of total rate of applied N, proportion of N applied at planting,
split applications of N, and N source on yield of 'Jubilee' sweet corn, NWREC, 1998.
Total N applied   N at planting    Yield     No. of   Mean ear  Tipfillz  Ear length 
lb/acre              lb/acre     tons/acre  ears/plot  wt. (g)             inches   
  0                      0          6.3        55        179      1.8        7.6     
 60                     40y         8.9        59        237      2.7        8.3
120                     40x         9.7        62        246      2.4        8.1
180                     40w         9.8        61        251      2.9        8.4
180                     80v        10.0        61        257      2.9        8.4
180                    120u         9.5        61        244      2.7        8.5
180                    180          9.5        61        245      2.8        8.5
                 LSD (0.05)         1.0         5         26                 0.1    
z5-point scale with 5 = perfect fill, 1 = 2 or more inches unfilled.
ySidedress N source: Greenfeed 27-0-0, 10 lb/acre, each, on 22 July and 12
August.
xSidedress N source: Greenfeed 27-0-0 and urea.  20 lb/acre each of Greenfeed and
urea applied on 22 July, 20 lb/acre as Greenfeed on 29 July and 12 August.
wSidedress N source: urea, applied at 60 lb/acre on 22 July, 40 lb/acre on 29 July,
and 40 lb/acre on 12 August.
vSidedress N source: urea, applied at 40 lb/acre on 22 July, 40 lb/acre on 29 July,
and 20 lb/acre on 12 August.
uSidedress N source: urea, applied at 20 lb/acre, each, on 22 July, 29 July, 12
August.


Table 11. Effects of total rate of applied N, proportion of N applied 
at planting, and split applications of N on post-harvest soil nitrate
and ammonium concentrations, NWREC, 1998.                              
N rate, lb/acre     Soil nitrate-N, ppm    Soil ammonium-N, ppm         
  0                         2.6                     4.8
 60                         2.9                     3.5
120                        11.8                     6.3
180                        37.6                     5.7
240                        40.8                     9.4 
180z                       20.5                     7.9
180y                       16.7                     6.4
180x                       21.9                     9.3
       LSD (0.05)          13.5                      NS               
zSidedress N source: urea, applied at 60 lb/acre on 22 July, 40 
lb/acre on 29 July and 40 lb/acre on 12 August.
ySidedress N source: urea, applied at 40 lb/acre on 22 July, 40 
lb/acre on 29 July, and 20 lb/acre on 12 August.
xSidedress N source: urea, applied at 20 lb/acre, each, on 22 July, 
29 July, 12 August.


Table 12. Main effects of rate of applied N and cultivar on SPAD chlorophyll
measurements in sweet corn, NWREC, 1988.                                                  
                                      Date of SPAD measurement                            
           16 July  22 July  27 July  3 Aug.  10 Aug.  17 Aug.  24 Aug.  1 Sept.  15 Sept.
N rate, lb/acre
  0           42.1     40.8     46.8    45.5     44.0     40.1    37.9    36.1     29.0  
 60           42.6     41.5     50.7    48.9     49.2     44.1    45.8    41.8     34.5
120           43.0     43.4     50.1    51.1     52.5     48.1    50.0    48.6     44.4
180           42.8     42.5     51.5    51.5     50.7     49.8    50.9    48.6     45.3 
240           42.0     41.4     51.8    51.7     50.2     48.2    50.5    48.7     43.7
  Significance NS       NS       ***     ***      ***      ***     ***     ***      ***  

Cultivar
Jubilee       40.0     38.5     46.3    45.8     44.1     41.4     41.8   39.1     33.1
GH 1703       44.9     45.3     54.0    53.7     54.5     50.7     52.2   50.4     45.6
  Significance ***      ***      ***     ***      ***      ***      ***    ***      ***   
***,NSSignificant at 0.1% level and nonsignificant, respectively.


Table 13. Effects of total rate of applied N, proportion of N applied at planting, split 
applications of N, and N source on SPAD chlorophyll measurements in 'Jubilee' sweet corn, NWREC, 1998.    
Total N         N at planting                           Date of SPAD measurement                          
applied, lb/acre   lb/acre   16 July  22 July  27 July  3 Aug.  10 Aug.  17 Aug.  24 Aug.  1 Sep.  15 Sep.
  0                   0       39.9     37.4     42.5     41.7     37.8     33.9     29.9     29.2    20.4
 60                  40z      40.9     39.8     46.7     45.7     43.9     40.0     40.7     35.3    31.0
120                  40y      38.5     37.7     46.1     45.2     43.9     42.6     44.6     41.7    37.7
180                  40x      40.3     38.7     45.5     45.9     42.2     42.3     43.9     42.7    38.3
180                  80w      39.2     39.5     46.9     45.6     42.5     41.6     43.7     42.8    38.4
180                 120v      39.8     40.5     48.3     47.7     44.3     41.5     41.9     41.5    37.5
180                 180       37.6     41.1     48.6     46.6     43.8     42.0     42.8     40.5    36.8
  LSD (0.05)                    NS      2.9      2.6      3.0      4.1      4.6      5.3      4.7     4.1
zSidedress N source: Greenfeed 27-0-0, 10 lb/acre, each, on 22 July and 12 August.
ySidedress N source: Greenfeed 27-0-0 and urea.  20 lb/acre each of Greenfeed and urea applied on 22 
July, 20 lb/acre as Greenfeed on 29 July and 12 August.
xSidedress N source: urea, applied at 60 lb/acre on 22 July, 40 lb/acre on 29 July, and 40 lb/acre on 
12 August.
wSidedress N source: urea, applied at 40 lb/acre on 22 July, 40 lb/acre on 29 July, and 20 lb/acre on 
12 August.
vSidedress N source: urea, applied at 20 lb/acre, each, on 22 July, 29 July, and 12 August.


Table 14. Sweet corn yield and soil and stalk nitrate-N concentrations as
affected by N rate, on-farm PSNT trial, 1997.                                     
           N fertilizer             Soil nitrate-N           Stalk     Corn
       Total rate Sidedress  Preplant     PSNT    Harvest  nitrate-N  yield
Grower ------lb/acre-------  --------------ppm---------------------- tons/acre   
A          130        70        9.1       13.5       4.9      3010     9.48
A          130        70        9.1       10.9       4.5     10000     9.09
A          130        70        9.1        8.8       4.5      9010     8.84
A          200       140        9.1       13.1      11.0      9550     9.53
A          200       140        9.1       10.0      11.7     11180     9.05
A          200       140        9.1       12.2       8.9     11880     9.42
B          135        85       22.0       33.3      20.1     13080     9.24
B          230       190       22.0       32.6      34.1     12120     9.31
C          116        64       38.2       35.4       8.9      2398     8.96
C          180       123        8.2       38.8      19.0      7129     8.89
D          140        80        7.3       15.6       7.5      8130     7.17
D          220       160        7.3       13.1       7.3      6250     6.97
E          140        88       10.4       22.9       8.3      8545    10.30
E          140        88       10.4       23.5       8.6      5801     8.94
E          140        88       10.4       25.4      14.0      6935     8.95
E          200       148       10.4       23.2      21.6      7501    10.59 
E          200       148       10.4       26.7      21.5      7161     9.63
E          200       148       10.4       27.0      12.7      7143     9.35
F          107        56       13.8       23.6       6.5      4926    10.48
F          107        56       13.8       22.9       7.2      4035    10.28
F          107        56       13.8       22.5       8.5      3144    10.07
F          145        94       13.8       21.2      13.2      4003    10.72
F          145        94       13.8       23.3       5.9      2904    10.08
F          145        94       13.8       21.1      13.2      2983    10.67
G          120        80       14.4       22.5       6.4      5372    10.65
G          200       160       14.4       19.9       2.5     10275    12.10
H          130        64        8.5       15.9       1.7       223    11.34
H          195       129        8.5       15.0       5.3      1574    11.83
I          140        90        1.4       12.2      12.1      7440     8.08
I          227       177        1.4       24.7      24.7     10950     9.66   



Table 15. Sidedress N rate for sweet 
corn based on 1995­1996 PSNT trials. 
PSNT           Sidedress N
ppm              lb/acre                        
<18                150
18 to 22           100
>22              50 to 80            



Table 16. Fertilizer, PSNT, yield, and soil and corn stalk nitrate-N concentrations in grower
fields, 1998.                                                                                    
Grower        N at    PSNT PSNT    N applied   PSNT plus    Yield   Relative  Nitrate at harvest 
             planting              sidedress  sidedress N             yield      Stalk   Soil
             lb/acre  ppm  lb/acre  lb/acre      lb/acre   tons/acre    %         ppm     ppm   
A   field      40      43   155       90           245       10.52                 --     20
    plot       40      35   125       45           170       10.82    102.9        --     19
B   field      66      27    97      116           213       13.47               12916    17
    plot       66      27    97       88           185       13.98    103.8      11729    19
C   field      40      32   115       40           155       11.56               11637    22
    plot       40      32   115       25           140       10.00     86.5      11921    41
D   field      47      15    53      155           208       11.22                3014    11
    plot       47      17    60      120           180       10.58     94.3       2370    12
E   field      50      25    88      160           248       10.05                7306    48
    plot       50      24    85       80           165        9.28     92.3       7015    20
F   field      39      39   142      120           262       11.72                9990    37
    plot       39      35   126       48           174       11.64     99.3      10890    41
G   field      45      33   117      160           277        7.55                2461    19   
    plot       45      37   133        0           133        7.24     95.9       4047    52
H   field      50      22    79      150           229       10.44                8197    17
    plot       50      21    76      100           176        9.67     92.6       6637    10
I   field      30      44   160       75           235        8.63                8923    67
    plot       30      43   155       50           205        8.48     98.3       8117    23    


Effect of Winter Cover Crops on Vegetable Crop Yield and Leaching of Nitrate

Cooperators: Dr. John Selker, Dept. of Bioresources Engineering, and Dr. Richard Dick, Dept. of Crop and Soil Sciences, Oregon State University

Introduction

Nitrate pollution of groundwater from the application of high rates of N fertilizers to vegetable crops is a concern in the Willamette Valley. Excess N not taken up by the crop remains in the soil and can be leached to groundwater during the wet winter months. Such concerns led us to initiate in 1990 a study of the cycling and availability of N in vegetable cropping systems. These are the eighth and ninth years of a study in which winter cover or "catch" crops have been seeded following vegetable crops and in which the N uptake of the cover crop and its contribution to a succeeding vegetable crop has been measured in comparison to a winter-fallow control. In 1994, sweet corn was grown on these long-term rotation plots at NWREC and fertilized at three rates of N. Following harvest the plots were seeded to cereal rye or a mixture of cereal rye and Austrian winter pea. In 1995, broccoli was grown on these plots at three rates of N to determine the cover-crop contribution to broccoli yield and N uptake. Following harvest, the plots were again disked, harrowed, and seeded (drilled) to triticale or a mixture of triticale and Austrian winter pea. In addition, other plots in both years were overseeded (relay intercropped) to cereal rye, triticale, or red clover about one month after sweet corn or broccoli emergence. These cover crops were permitted to grow through the winter. In 1996, sweet corn was again grown on the plots and fertilized with three rates of applied N.

In autumn 1993, passive capillary wick samplers were installed beneath the winter-fallowed plots and fall-planted cereal rye (later triticale) plots. All three N rates were also represented. The samplers have allowed us to collect leachate on a continuous basis and determine both the nitrate concentration of the leachate as well as the total nitrate loss on an area basis. Details of the installation and use of the capillary wick samplers can be found in Brandi-Dohrn et al., 1997.

Objectives in 1997 and 1998 were 1) to evaluate effects of several winter cover crops, including fall-seeded and overseeded triticale, fall-seeded triticale plus winter pea, and overseeded red clover on yield and quality of broccoli (1997) and sweet corn (1998) at three rates of N and 2) to evaluate the effect of these cover crops and the N applied to the vegetable crops in 1996 and 1997 on the amount of nitrate leached below the root zone.

Methods, Broccoli, 1997

During winter, the plots had been fallow or in the cover crops listed under Objectives. The cover crops had been overseeded into standing sweet corn in July 1996 or were broadcast-seeded and scratched into the soil in early October, 1996. While we did not consider this an outstanding cover crop in terms of biomass accumulation, the plots seeded to cover crops in October had significantly (P=0.01) greater ground cover than did fallow plots, when measured in January (Table 1).

'Packman' broccoli was seeded on 3 July in rows 30 inches apart. Two previous seedings of 'Pirate' failed because of inadequate stands. Plot size was 600 square feet. Nitrogen rates were 0, 125, and 250 pounds/acre, with half the N applied just after seeding, and the remainder applied 5 weeks after seeding. At this time, the appropriate plots were overseeded to 'Celia' triticale or 'Kenland' red clover in preparation for the 1998 experiments. Harvest was on 16 September.

Methods, Sweet Corn, 1998

During winter the plots had been fallow or in the cover crops listed under Objectives. The cover crops had been overseeded into the standing broccoli crop in July 1997 or were broadcast-seeded and harrowed into the soil in early October, 1997. 'Jubilee' sweet corn was seeded on 1 June in rows 30 inches apart. The stand was thinned to 6­7 inches between plants in the row. Plot size was 600 square feet. Nitrogen rates were 0, 50, and 200 lb/acre, with half the N applied just after seeding and the remainder applied 5 weeks after seeding. At this time the appropriate plots were overseeded to common cereal rye or 'Kenland' red clover in preparation for the 1999 experiments. Harvest was on 9 September.

Results, N Rate and Cover Crop on Broccoli Yield, 1997

Both yield and mean head weight tended to decline following an overseeded triticale cover crop, regardless of N rate (Table 2). This result is consistent with those dating back to 1990 for both triticale and cereal rye cover crops (Hemphill, 1993, 1995, 1997). The overseeded clover cover crop tended to increase yield but the effect was not statistically significant. However, mean head weight was significantly increased with the overseeded clover. Yield with fall-seeded triticale tended to be higher than following fallow, and mean head weight was significantly improved by the fall-seeded triticale. In past years, fall-seeded cereal cover crops have tended to depress yield. Results from this year may indicate that soil available N content has increased sufficiently with continuous cover- cropping to provide some yield boost to the following vegetable crop. Initial pea stand in the triticale/pea cover crop was adequate, but the stand was lost during the late winter. Consequently, yield did not increase with this cover crop over that obtained with triticale alone. There was no response to the rate of N applied when averaged over cover crop treatments. The highest-yielding treatment combination was the intermediate rate of applied N following the fall-seeded triticale/pea cover, with 5.2 tons/acre (Figure 1). Largest mean head size was obtained with the combination of fall-seeded triticale and no applied N (Figure 2). Lowest yield and head weight were with overseeded triticale and no applied N.

Figs. 1 and 2

Winter Cover Crop on Nitrate Leaching, 1996-1997

A fall-seeded triticale cover crop significantly reduced the nitrate concentration of leachate reaching the 4- foot depth in the soil profile following the 1996 corn crop (Figure 3). The difference in nitrate-N concentration in the leachate was small during the high-rainfall months of December and January, but the amount of water percolating past the root zone was also reduced by the cover crop, resulting in a dramatic effect on the amount of nitrate being leached (Figure 4). By April, the amount of nitrate lost from the root zone was 33 pounds N/acre for the fallow plots versus 18 pounds/acre for the cover crop plots at the high rate of N applied to the previous vegetable crop. This is very consistent with results obtained in the winters of 1992­93 through 1995­96 (Hemphill, 1995, and 1997). Over the five winters that we have obtained data from the passive capillary lysimeters, the reduction in mass of nitrate leached has averaged 45 percent. This demonstrates the ability of even a relatively sparse cover crop to significantly improve ground and surface water quality.

Figs. 3 and 4

Cover Crop Yield and N Uptake, 1997­1998

While the cover crops, again, were rather sparse and slow-growing, all plots seeded to cover crops had significantly (P=0.01) greater ground cover than did fallow plots when measured in February (Table 3). In addition, most of the biomass on fallowed plots was attributable to annual bluegrass, which is shallow-rooted and does not recover much N. Cover-crop biomass accumulation and nitrogen uptake were below average for these plots, but some interesting trends emerged. The yield of overseeded crops was generally larger than for the fall-seeded crops, a reversal of the previous trend (Table 4). This may have been due to the late planting date of the fall-seeded crops, saturated soil during much of the winter, and a soil pH which was getting lower than desired. Except for the overseeded red clover, cover-crop biomass and N accumulation did tend to increase with increasing rate of N applied to the preceding broccoli crop.

N Rate and Cover Crop on Sweet Corn Yield, 1998

Although not statistically significant, yield, mean ear weight, ear length, and tipfill tended to decline following an overseeded triticale cover crop, regardless of N rate (Table 5). This result was consistent with those dating back to 1990 for both triticale and cereal rye cover crops. In past years, fall-seeded cereal cover crops have also tended to depress yield, but this was not the case in 1998, perhaps because of the poor stand and growth of the fall-seeded triticale. Initial pea stand in the triticale/pea cover was adequate, but the stand was lost during the late winter because of a root disease. Therefore, yield did not increase with this cover crop over that obtained with triticale alone. The response to rate of N applied was normal when averaged over cover crop treatments. The highest-yielding treatment combination (10.0 tons/acre) was the high rate of applied N following the overseeded clover cover, indicating some N contribution from the clover, despite the relatively low N uptake (Table 3) of this cover. This treatment combination also produced the largest mean ear size. Lowest yield and mean ear weight was with overseeded triticale and no applied N.

Winter Cover Crop on Nitrate Leaching, 1997-1998

Averaged over rates of N applied to the preceding broccoli crop, a fall-seeded triticale cover crop significantly (P=0.05) reduced the nitrate concentration of leachate reaching the 4-foot depth in the soil profile for two sampling dates and tended to do so for all but the last sampling date (Table 6). The difference in nitrate-N concentration in the leachate between fallow and covered plots was larger during the fall and early winter months than in late winter. In fact, at the high rate of applied N, but not at the zero and intermediate rates, nitrate concentrations were higher in leachate collected beneath the triticale cover crop than below fallow plots for the last four sampling dates (Figures. 5­7). This is a reversal of the trends seen in previous years (Hemphill, 1997). In past years, nitrate concentrations of collected leachate have varied up and down during the rainy season but in 1997­ 1998, the concentrations tended to increase steadily during the winter. The reason for this is not known, but perhaps significant mineralization of organic N was occurring during the mild winter.

Figs. 5-7

Summary

Consistent with past results, winter cover crops reduced leaching of nitrate from the root zone in the winter of 1996­1997. Leguminous cover crops made N available to the following vegetable crop, but a cover crop consisting only of an overseeded winter grain tended to depress yield of the following broccoli crop. In contrast to previous years, a fall-seeded triticale cover crop tended to increase broccoli yield.

In the winter of 1997­1998, a triticale cover crop reduced nitrate concentrations in leachate consistently at the zero and intermediate rates of applied N, but not at the high rate of N. This is in contrast to results for the previous five winters. A clover cover crop made N available to the succeeding sweet corn crop in 1998, but a cover crop consisting only of an overseeded winter grain tended to depress yield of the following sweet corn crop.

Literature Cited

Brandi-Dohrn, F.M., R.P. Dick, M. Hess, S.M. Kauffman, D.D. Hemphill, Jr., and J.S. Selker. 1997. Nitrate leaching under a cereal rye cover crop. J. Environ. Qual. 26:181­188.

Hemphill, D.D., Jr. 1993. Vegetable research at the North Willamette Research and Extension Center, 1991­1992. Oregon Agric. Expt. Sta. Spec. Rep. No. 908.

Hemphill, D.D., Jr. 1995. Vegetable research at the North Willamette Research and Extension Center, 1993­1994. Oregon Agric. Expt. Sta. Spec. Rep. No. 944.

Hemphill, D.D., Jr. 1997. Vegetable research at the North Willamette Research and Extension Center, 1995­1996. Oregon Agric. Expt. Sta. Spec. Rep. No. 975.

Table 1. Effect of cover crop on percentage of ground covered, as
determined by the string method, 6 January, 1997.                    
Cover crop              % Triticale    % Legume    % Weeds    % Total        
Fallow                       0             1          34         35            
Overseeded triticale        12             0          30         42
Overseeded clover            0            21          25         46
Fall-seeded triticale       36             1          30         67
Fall-seeded triticale/pea   20            29          18         66
                LSD (0.05)  14             7          15         17            


Table 2. Main effects of preceding cover crop and rate of
applied N on yield of broccoli, NWREC, 1997.                
Treatment                          Yield    Mean head wt.                
                                 tons/acre       (g)        
Cover crop (avg. over N rates)      
Fallow                              3.5         349
Overseeded triticale                2.6         236
Overseeded clover                   4.3         407
Fall-seeded triticale               4.1         466
Fall-seeded triticale/pea           4.4         408
                        LSD (0.05)   NS          22 
N rate, lb/acre (avg. over covers)
  0                                 3.8         383
125                                 4.0         375
250                                 3.9         361
                        LSD (0.05)   NS          NS              


Table 3. Effect of cover crop on percentage of ground covered, as
determined by the string method, 4 February, 1998.                   
Cover crop              % Triticale    % Legume    % Weeds    % Total 
Fallow                       0             0          34         34      
Overseeded triticale        28             9          51         88
Overseeded clover            0            28          68         96
Fall-seeded triticale       26             2          25         53
Fall-seeded triticale/pea   34            19          21         74
                LSD (0.05)  12             9          12         10  


Table 4. Interaction of cover crop and rate of N applied to preceding 
broccoli crop on cover crop biomass and N uptake, NWREC, 1998.            
Cover crop             N rate    Cover dry biomass    N uptake         
                       ----------------lb/acre----------------
Overseeded triticale         0          1712             26
                           125          3090             46
                           250          3759             58
Overseeded clover            0          1893             46
                           125          2141             37
                           250          2249             38
Fall-seeded triticale        0           796             17
                           125           743             16
                           250          1128             28
Fall-seeded triticale/pea    0           716             13
                           125           607             10
                           250          1054             20
                         LSD (0.05)      700             14             


Table 5. Main effects of preceding cover crop and rate of applied N on     
yield of sweet corn, NWREC, 1998.                                               
Treatment                          Yield    Mean ear wt.  Ear length  Tipfill  
                                 tons/acre      (g)        (inches)            
Cover crop (avg. over N rates)                                            
Fallow                              7.5         203          8.2        2.1
Overseeded triticale                5.6         164          8.0        1.6
Overseeded clover                   7.3         210          8.1        1.9
Fall-seeded triticale               7.5         210          8.1        2.3
Fall-seeded triticale/pea           7.0         198          8.1        2.2
                        LSD (0.05)   NS          NS           NS         NS
N rate, lb/acre (avg. over covers)
  0                                 4.5         140          7.3        1.2
125                                 7.6         206          8.2        2.0
250                                 8.9         245          8.8        2.9
                        LSD (0.05)  0.8          20          0.2        0.3   


Table 6. Main effects of rate of N applied to 1997 broccoli crop and a 
triticale cover crop on nitrate concentration of leachate collected during 
the 1997­1998 rainy season.                                                   
                                     Collection date                         
             Nov. 5   Dec. 3   Dec. 19  Jan. 9  Jan. 21  Feb. 13   Mar. 24
             -----------------------ppm Nitrate-N-------------------------
Cover crop
Triticale       3        4        6        7      10       15        20   
None            6        7        9        9      12       16        18
 Significance  NS       **        *       NS      NS       NS        NS
 
N rate, lb/acre
  0             3        3        3        4       4        6         7
125             4        4        6        6       9       13        16
250             8       10       13       15      19       28        34
 Significance   *       **       **       **      **       **        **      
**,*,NSSignificant differences at 1% and 5% probability levels, and no 
significant differences, respectively.

Impact of Cover Crops on Mold Incidence in Snapbeans

COOPERATOR: Dr. Mary Powelson, Dept. of Botany & Plant Pathology, Oregon State University

Introduction

Limited fungicide options and the tentative state of continued registration of vinclozolin for snapbeans require development of additional management strategies for suppression of white mold (Sclerotinia sclerotiorum). One encouraging technique for suppression is to eliminate or reduce spring tillage and leave a straw mulch derived from a cover crop. In 1993, weed emergence was greatly reduced and white mold incidence was reduced nearly 98 percent when beans were planted through a barley cover-crop residue. Snapbean yields were not affected by the cover-crop residue. This mold reduction may have been due to the presence of the barley residue acting as a physical barrier between the white mold apothecia and the bean canopy. In addition, several compounds that have antifungal properties have been isolated from both cereal and cole crop residues. Crop residues may also influence the survivability of sclerotia by stimulating soil microbes which act as predators of sclerotia. Alternatively, actively growing cover crops may induce germination of the sclerotia before bean planting and reduce survivability of the sclerotia.

Integrated management of snapbean diseases depends on the availability, understanding, and potential application of several concurrent strategies. Both white and grey mold are diseases that must be effectively controlled. Our past research has indicated that both white and grey mold development may be affected by changes in tillage systems, vegetative management, and the preceding cover crop or cash crop.

The objective for 1997 was to evaluate the impact of a cover crop and the preceding vegetable crop on the incidence of white mold and grey mold in snapbean.

Methods

A field was divided into 15 ´ 60-foot plots of 'Jubilee' sweet corn, 'Gem' broccoli, or summer fallow during summer 1996. White mold sclerotia were buried in mesh bags in each plot. Sclerotia survival was extremely low. In the late summer of 1996, these plots were flailed and disked and then split by a winter fallow treatment or a cover crop of triticale plus Austrian winter pea. Treatment combinations were replicated five times. In late May 1997, after taking samples for biomass, the cover crops were mowed and disked several times, and the plots were seeded to Oregon 91 beans, with three rows/5-foot bed. No herbicides were used. A total of 160 pounds N/acre was applied to the bean crop, which along with the high seeding rate, created favorable conditions for development of white mold. Laboratory-grown sclerotia were applied to 9-square-foot sections at the center of each plot. White and grey mold ratings were made on 18 September, and plots were harvested on 24 September, 1997.

Results

Greater cover-crop/weed biomass accumulated on plots which had been in broccoli or summer fallow compared to those which had been in sweet corn (Table 1). Although herbicide residue may have played a role in this effect, the most likely explanation is that corn residue interfered in the drilling of the cover crop. Sweet corn dry biomass was 6.3 tons/acre compared to 2.2 tons/acre for broccoli. Bean yield and number of plants/plot did not vary significantly with previous crop/cover crop.

The number of plants with white mold also did not vary with treatment although there was a tendency for higher white mold incidence in plots planted into cover crop residue. The number of infected pods recovered per plot also tended to be higher following a cover crop. The previous vegetable crop had no influence on white mold. Expressed as a percentage of the total number of plants/plot, treatments did not affect white mold. The cover-crop residue obviously did not provide a barrier between sclerotia on the soil surface and the bean plants. This may be because the sclerotia were applied after planting and may not have remained in or on the residue. It is also doubtful that the white mold incidence was related to sclerotia applied to the plots: incidence did not appear to be higher in the portion of the plot that had sclerotia applied. The high plant density, rank growth, and over-irrigation probably caused the high incidence of white mold in this study. A straw residue on the surface might actually have favored white mold development by contributing to greater surface moisture and humidity in the plant canopy.

The number of plants infected with grey mold was decreased in plots that had been in broccoli the previous summer and tended to be increased in plots with cover crop residue. The number of infected pods per plot also tended to decrease following broccoli and to increase following the cover crop. The percentage of plants affected by grey mold was more than halved following broccoli.

Table 1.  Main effect of previous vegetable crop and winter cover crop on yield,
white mold incidence, and grey mold incidence in snapbean, NWREC, 1997.                  
Treatment    Cover dry    Yield        White mold incidence        Grey mold incidence  
             mass, g/m2  tons/acre  # plants  % plants  # pods  # plants  % plants #pods
________________________________________________________________________________________
1996 crop
Summer fallow   302        11.4       17.3      22.2      29.5     8.8      12.3   10.2 
Sweet corn      180        13.1       19.5      29.2      22.1     8.3      12.3    9.4
Broccoli        245        11.3       19.5      28.2      20.9     3.9       5.4    4.7
    Significance *          NS         NS        NS        NS       *         *      NS
Cover crop
None (weeds)     89        12.0       14.6      20.3      19.1     5.5       8.4    6.3
Triticale/pea   362        11.8       23.2      32.7      29.3     8.5      11.6    9.9
   Significance  **         NS         NS        NS        NS       NS       NS      NS 
**,*,NSSignificant at 1% and 5% levels, and non-significant, respectively.


Cultivar, N Rate, and Between-Row Spacing on Yield of Edamame

Cooperator: Dr. Carol A. Miles, Cooperative Extension, Washington State University

Introduction

Edamame or vegetable soybean (Glycine max [L.] Merrill) is a specialty soybean that is harvested as a vegetable when the seeds are still immature. It is usually sold as pods or as whole harvested stems but occasionally as shelled beans. The seeds are usually boiled in the pod, shelled out, and eaten as a snack, as a vegetable with meals, or added to soups or confections. When eaten as a vegetable, the seeds are added to salads, stir-fried, or combined into vegetable medleys. As a sweet, the beans are ground to a paste, sweetened, and used as a topping for sticky rice. Most production and consumption is in China, Japan, Taiwan, and Korea. Most production research, variety development, and production guides originate in Taiwan and Japan.

Washington State University has conducted variety trials and is involved in a breeding program for improved varieties of edamame for the Pacific Northwest. Very little information on production techniques for the coastal areas of the Northwest is available. Information derived from the Asian literature is often an inadequate guide to production in our area. For example, the Japanese literature recommends application of 35 to 55 pounds N/acre in addition to 8 tons/acre of well-decomposed animal-waste compost which provides an unknown amount of available nutrients (O'Rourke, 1994). Recommendations for plant populations are not consistent. If this crop is to be grown in the Pacific Northwest, which appears to have a good climate for production of high yields and quality, we will need to develop recommendations based on the soil types and cultural methods likely to be used in this region. The purpose of this trial was to investigate the effects of two, between-row spacings and three rates of applied N on three edamame cultivars previously determined by Carol Miles to have the potential for high yield west of the Cascades.

Methods

Non-inoculated 'Butterbeans', 'Shironomai', and 'White Lion' edamame were seeded to a winter-fallowed Willamette silt loam, pH 5.8, at the NWREC on 3 June. Plot preparation included plowing, disking, a broadcast and incorporated application of triple superphosphate and sulfate of potash, each at 200 pounds/acre, and harrowing to form a seedbed. The three cultivars were seeded on 20 and 30-inch rows, with 3-inch in-row spacing. Metolachlor, 1.5 pounds/acre, was applied after planting for weed control. Escaped weeds, mostly sowthistle, prickly lettuce, groundsel, and red-root pigweed, were controlled by hand-hoeing. Nitrogen rates applied were 0, 36, and 72 pounds/acre as ammonium nitrate, with half the N applied one week after seeding and the remainder on 8 July, near first bloom. The experimental design was a randomized complete block split plot, with cultivar ´ spacing combinations as main plots and N rates as subplots. Subplot size was 15 ´ 20 feet. Plots were sprinkler irrigated as necessary, usually approximately one inch/week. Yields were estimated by harvest of a 10-foot section of one of the centermost rows of each subplot. Pods were stripped by hand, separated into categories of two or more beans/pod, one bean/pod, and unmarketable (mostly lacking developed beans). Cultivars differed in maturity with 'White Lion' harvested on 8 September, 'Shironomai' on 16 September, and 'Butterbeans' on 21 September.

Results

Cultivar. The cultivars differed significantly in plant development (Table 1). Stand of 'Shironomai' was nearly 30 percent less than that of 'Butterbeans' or 'White Lion'. 'Butterbeans' and 'White Lion' produced plants from about 63 percent of the planted seed, while the establishment rate for 'Shironomai' was only 44 percent. 'White Lion' produced a slightly shorter and narrower canopy (measured 30 July) and bloomed earlier than the other two cultivars. Canopy height of 'White Lion' did not increase with increasing rate of N to the same extent as for the other cultivars (Table 2).

Despite its relatively poor stand, 'Shironomai' produced a higher yield/acre, higher yield of large pods (2 or more beans), and larger pod weight/plant than did the other cultivars (Table 3). A greater pod weight/plant more than offset the reduced stand. 'Shironomai' also tended to have the heaviest pods. 'White Lion' produced the smallest total and marketable yield.

Although there were significant interactions of cultivar and N rate affecting total pod weight/plant and marketable pod weight/plant (Table 4), 'Shironomai' was the highest yielding cultivar at each N rate. The interaction of between-row spacing and cultivar also affected total and marketable pod weight/plant (Table 5) but 'Shironomai' was the highest yielding at both spacings.

Spacing. The 20-inch spacing produced a greater stand per meter of row than did the 30-inch spacing (Table 1). This is because one of the two planters per bed was not properly covering seed and stand was reduced on this row for the 30-inch spacing. With 20-inch spacing, only one of three planters did not adequately cover the seed, so mean stand was greater. The main effect of spacing on canopy development on 30 July and on bloom was not significant. However, spacing and N rate interacted to affect bloom (Table 6). Bloom was advanced at the lowest rate of N for 20-inch but not for 30-inch spacing.

The 30-inch spacing produced a higher yield/plot (per meter of row) but a lower yield on an area basis (Table 3). Mean weight of individual pods was higher at 30-inch spacing as was total and marketable pod weight/plant. But the nearly 50 percent higher number of plants/unit area at the 20-inch spacing offset the greater per- plant yield obtained with the wider spacing.

N Rate. Rate of N had no effect on stand, but canopy height and width increased linearly with increasing N (Table 1). Increasing N also delayed flowering.

Total weight harvested/plot, yield/unit area, weight of marketable pods/plot, mean pod weight/plant, and mean marketable pod weight/plant increased linearly with increasing rate of N. However, mean weight of the individual pod did not increase from the intermediate to the highest rate of N. The number of beans/pod, mean bean weight, and number of plants harvested per plot did not vary with N rate (Table 3).

Although the main effect of N rate on total and marketable pod weight/plant was a linear increase with increasing rate of N, not all cultivars were consistent in their response to N (Table 4).

There were no 3-way interactions of N rate, spacing, and cultivar affecting plant development or yield.

Conclusions and Discussion

For the purposes of this experiment, marketable yield was defined as those pods acceptable for fresh marketing in-pod. However, pods with only one bean may be marketable as shelled green edamame or as seed for planting. Yields of both one-bean pods and pods with 2 or more beans responded similarly to treatment.

Given that complete canopy closure was not obtained with the 30-inch spacing, that mean bean weight was not affected by spacing, and that mean pod weight was only slightly lower at the 20-inch spacing, 20 inches appears to be a reasonable between-row spacing for these cultivars on this Willamette soil. Although mean pod weight, number of beans/pod, and mean bean weight did not increase between the intermediate and high rates of N, yield/plant and per unit area did increase between the intermediate and high rates, indicating that 72 pounds/acre may not be sufficient for maximum production in a situation where the seed was not inoculated, the soil had not recently been used for production of legumes, and there was not a history of application of manures or composts. Although not quantified, we noticed a strong tendency for a high proportion of the plants to have nodules at the zero N rate, but not at other N rates. These plants, although dark green in color, did not exceed non-nodulated plants in size. Inoculated seed might have responded differently to N rate.

Literature Cited

O'Rourke, A.D. 1994. Edamame: the vegetable soybean. p. 173­199. In: A.D. O'Rourke (ed.). Understanding the Japanese food and agrimarket, a multifaceted opportunity. Haworth Press, New York.

Table 1. Main effects of cultivar, between-row spacing, and rate of applied N
on stand, plant height and width, and flower development in edamame, NWREC, 1998. 
               Seedlingsz/  Canopy heighty   Canopy widthy      Plants in bloom    
                   6 m          inches          inches    23 July  30 July  6 Aug.
Cultivar                                                   ----------%----------
Butterbeans       49             19             17           0       32      96
Shironomai        35             19             17          26       15      97
White Lion        50             17             15           0       73      99
  Significance    ***            ***            ***         ***      ***     NS

Spacing
20 inches         48             18             16           9       44      98
30 inches         42             18             17           8       35      98
  Significance    ***            NS             NS          NS       NS      NS

N rate, lb/acre
 0                45             17             15          12       52      99
36                44             18             17           7       35      98
72                45             19             18           8       32      96
  Significance    NS             ***            ***         NS       ***     NS    
***,NSSignificant at 0.1% level and nonsignificant, respectively.
zCounts made 18 June.
yMeasured 30 July.


Table 2. Interaction of cultivar and rate of applied N, averaged 
over two spacings, on canopy height of edamame, NWREC, 1998.       
Cultivar     N rate, lb/acre   Canopy height, inches             
Butterbeans          0                  16.1
                    36                  19.5
                    72                  19.9
Shironomai           0                  17.9
                    36                  18.9
                    72                  19.4
White Lion           0                  16.4
                    36                  16.5
                    72                  17.0
    LSD, Significance                    1.0***                     
***Significant at 0.1% level of probability.


Table 3. Main effects of cultivar, between-row spacing, and rate of applied N fertilizer on edamame yields, 
NWREC, 1998.                                                                                                
             Total wt.  Unfilled  1 bean/  2+ beans/  Wt. 100  No. of    Mean    No. of  Pod wt./ Marketable
              g/  tons/   pods     pod       pod       pods    beans/  bean wt.  plants/  plant   pods/plant
             plot acre   g/plot   g/plot    g/plot      g      25 pods     g       3 m      g         g     
Cultivar
Butterbeans  1353  3.1     56      433       864       259       57      0.60     26.5     53         34
Shironomai   1551  3.6    137      431       974       278       54      0.52     17.7     91         58
White Lion   1136  2.6     56      340       718       251       NR       NR      25.0     46         29
 Significance ***  ***    ***       **       ***        NS        *       *        ***     ***        ***

Spacing  
20 inches    1154  3.3     60      338       745       249       56      0.55     22.1     54         35
30 inches    1539  2.9    106      464       958       276       56      0.58     24.0     72         45
 Significance ***   **    ***      ***       ***        *        NS       NS       NS      ***        **

N Rate, lb/acre
 0           1101  2.5     42      325       726       251       57      0.55     21.8     53         35
36           1400  3.2     86      433       874       269       55      0.57     24.5     65         41
72           1539  3.5    120      446       956       269       56      0.56     22.9     71         45
 Significance ***  ***     **      ***        **        *        NS       NS       NS      **          *    
*,**,***,NSSignificant at 5, 1, and 0.1% probability level, and non-significant, respectively.


Table 4. Interaction of rate of applied N and cultivar, averaged 
over two spacings, on mean pod weight per edamame plant, NWREC, 1998.            
Applied N, lb/acre   Cultivar    Total pod wt./plant  Marketable pod wt./plant
                                 ---------------------g-----------------------
 0                   Butterbeans        51                      36  
                     Shironomai         70                      44
                     White Lion         38                      24
36                   Butterbeans        44                      27
                     Shironomai        105                      65
                     White Lion         48                      30
72                   Butterbeans        63                      38
                      Shironomai       100                      63
                     White Lion         51                      32
     LSD, Significance                  16*                     11*           
*Significant at 5% level.


Table 5.  Interaction of between-row spacing and cultivar, averaged over three
rates of applied N, on the mean pod weight per edamame plant, NWREC, 1998.       
Row spacing, inches   Cultivar   Total pod wt./plant  Marketable pod wt./plant
                                 ---------------------g-----------------------
20                    Butterbeans         47                  31
                      Shironomai          72                  45
                      White Lion          44                  29
30                    Butterbeans         59                  37
                      Shironomai         111                  70
                      White Lion          47                  29
  LSD, Significance                       13**                 9*             
**,*Significant at 1 and 5% levels, respectively.


Table 6. Interaction of between-row spacing and rate of applied N, averaged
over three cultivars, on flower development of edamame, NWREC, 30 July, 1998. 
Spacing, inches      N rate, lb/acre      Plants in bloom, %                  
20                          0                    64
                           36                    33
                           72                    35
30                          0                    39
                           36                    37
                           72                    29
      LSD, Significance                          15*                         
*Significant at 5% level.

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