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Vegetable Research at the North Willamette Research and Extension Center, 1993-1994

CONTENTS

Introduction
Pickling Cucumber Variety and Weed Control Trials
Rate, Timing of Application, Source, and Placement of Nitrogen Fertilizer on Yield of Cauliflower
Rate, Timing of Application, Source, and Placement of Nitrogen Fertilizer on Yield of Sweet Corn
Nitrogen Rate on Yield of Green Beans, Beets, and Carrots, and Residual Mineral Nitrogen Concentration of Willamette Silt Loam
Post-Harvest Mineral Nitrogen Status in Grower Fields
Cover Crops and N Rate on Yield of Sequential Crops of Broccoli and Sweet Corn
Irrigation Effects on Head Rot of Broccoli

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AUTHOR:

Dr. Delbert D. Hemphill, Jr., Professor of Horticulture, has conducted research on vegetable crops culture and management since 1976 at Oregon State University's North Willamette Research and Extension Center, 15210 NE Miley, Rd., Aurora, OR 97002-9543.

COOPERATORS:

Dr. N.S. Mansour is Extension Vegetable Specialist and Professor, Department of Horticulture, Oregon State University, Corvallis, OR 97331

Dr. John Hart is Extension Soil Scientist and Associate Professor, Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331

Dr. Richard Dick is Associate Professor of Soil Science, Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331

Dr. Mary L. Powelson is Professor, Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331

Mr. Robert B. McReynolds is District Extension Agent for vegetable crops and Associate Professor, North Willamette Research and Extension Center, 15210 NE Miley Rd., Aurora, OR 97002-9543

Dr. John Luna is Assistant Professor and On-Farm Research Coordinator, Department of Horticulture, Oregon State University, Corvallis, OR 97331


Introduction to the Report

We have conducted a full-time program of vegetable crop research at the North Willamette Research and Extension Center (formerly the North Willamette Experiment Station) since 1976. The Center, a branch of both the Oregon State University Agricultural Experiment Station and Cooperative Extension Service, is just north of Aurora, a historic farming community 20 miles south of Portland, Oregon. The land is provided by Clackamas County, with facilities owned and maintained by OSU. The Center serves the vegetable, small fruit, wine grape, and nursery crops industries and is located in an area noted for the diversity of its agriculture. Our vegetable research emphasizes the needs of both the fresh market and processed vegetable growers in the Willamette Valley. We also conduct research on home garden and small farm-intensive vegetable culture.

Many of the research projects reported here involved cooperation with Experiment Station and Extension Service colleagues at Oregon State University and their contributions are gratefully acknowledged. The financial support of the Oregon Processed Vegetable Commission, Oregon Department of Environmental Quality, Nalley Fine Foods, UNOCAL, the Center for Applied Agricultural Research, and the Agricultural Research Foundation was essential to completing these projects and is greatly appreciated.

This report is the ninth in a series of biennial reports initiated in 1979.

DISCLAIMER: The use of trade names does not constitute an endorsement by the Oregon State University Agricultural Experiment Station. Always check pesticide labels for currently registered uses.


Pickling Cucumber Variety and Weed Control Trials

Cooperators: N.S. Mansour, Dept. of Horticulture; Robert B. McReynolds, North Willamette Research and Extension Center

Introduction

Pickling cucumber growers and processors are interested in mechanical harvest of fruit to avoid problems with labor cost and availability. Mechanical harvest requires changes in varieties, number of plants/acre, and cultural practices. We have been conducting trials since 1989 to determine the feasibility of machine harvest in the Willamette Valley. This report focuses on the two most recent growing seasons. Our 1993 and 1994 trials included a number of varieties screened in earlier trials as well as some varieties not previously included, and on the effect of harvest date on maturity.

Varieties thought to have a good length/diameter ratio and processing quality were grown in commercial-scale trials. Each variety was harvested more than once to determine days or heat units to the optimum size distribution and the rate at which size grade increases with time. Pickling cucumbers are graded into four sizes by diameter (See Table 1), with the smaller sizes of greater value to the processor. Growers are paid considerably more per pound for the smaller sizes. "Size grade" is determined from the proportion by weight of fruit falling into each of the four size categories. As a field matures, the tonnage and size grade increase, while the value per ton decreases. Harvest and pricing policy must be adjusted both to fit processor pack needs and to provide adequate return for both processor and grower.

Weeds reduce yield and harvester efficiency. High weed populations may cause expensive downtime for cleaning the harvester pickup head. Chloramben (Amiben) and naptalam (Alanap) have been withdrawn from the market. Other herbicides have proven only partially effective or damaging to the crop. Curbit has found a niche with some growers but inconsistency in weed control and crop injury limits its use. Testing of alternatives has been conducted to identify combinations of herbicides that are effective and non-phytotoxic to the crop. The 1991 and 1992 trials indicated that clomazone (Command) shows promise. Weed control was excellent but there was some damage to the cucumber seedlings in 1991. It was apparent that more information on crop response to clomazone, alone or in combination with other materials, was still needed. A 1993 trial indicated insignificant phytotoxicity problems, but the field was so weed-free that no useful information on efficacy was obtained. This trial was repeated in 1994.

Methods

The variety trials were established in a commercial planting just south of Woodburn, Oregon. The previous crops were oats (1993) and sugar beets (1994). Planting date was 8 June, 1993, and 16 May, 1994, following typical land preparation by the grower. Each variety was planted to an area of slightly less than 1.2 acres. The area for each variety was divided into two approximately equal and separate replicates. The planter seeded six rows at a time on 30-inch centers. Planters were not adjusted for the slightly different seed size of each variety; thus, seeding rate varied somewhat with variety. The goal was to produce a stand of about 80,000 plants/acre.

The entire area received a broadcast, pre-plant application of 40 pounds N and 42 pounds K/acre and a banded application of 60 pounds N and 78 pounds P/acre at planting, for a total N application of 100 pounds/acre. This rate appears from our previous work to be optimal for machine-harvested cucumbers. Irrigation, cultivation, and herbicide application were provided by the grower.

We hand-harvested all varieties three times between 6 and 10 August, 1993, and 18 and 22 July, 1994, using a once-over destructive harvest for each 90-square foot plot. The fruit was weighed and graded on a Kerian 'SpeedSizer' mechanical grader provided by Nalley Fine Foods. The commercial plots of all varieties were harvested by Nalley's personnel using an FMC harvester on 10 August, 1993, and 22 July, 1994. A Byron harvester was also used for one variety in 1993. The machine-harvested samples were weighed and graded at the Nalley receiving station at Cornelius.

The 1994 weed control plots were located at the North Willamette Research and Extension Center to take advantage of the better control it offers for managing operations and a greater weed population. The first of two plantings ('Flurry-M') was direct-seeded with Massey-Ferguson International planters on 24 May, 1994. The herbicide treatments were selected to verify results from the previous year and to establish the lower limit at which Command would be effective when applied alone and in combination with Prefar. The preplant treatments were applied one day before planting with a CO2 backpack sprayer (40 psi) to a dry soil surface and incorporated to 3-inch depth with a PTO-driven power tiller. The pre-emergence treatments were applied immediately after planting and the entire area was then irrigated with approximately 1 inch of water.

The trial design was a randomized complete block with four replications. Four rows were planted to a 12 x 30 foot plot. Weed control ratings represent the mean rating of the three project leaders, each rating independently of the others. Plots were harvested on 3 August.

Commercial (Machine-Harvested) Variety Trial, 1993

It is unfortunate that the harvester could not be scheduled into the variety trial on more than one day. Since processors want to harvest at a mean size grade near 2.5, it is apparent that 'Duke', and to a lesser extent 'Flurry-M', 'Napoleon', and 'Quest', were harvested between one and two days after peak maturity (Table 1). The later-maturing 'Lafayette' and the Sun line were harvested at optimal maturity.

Fortunately, the Byron and FMC harvesters harvested several acres of 'Flurry-M' in the same field on 6 August. This gave a basis for comparison with the hand-picked samples on the same day, and a comparison of the two harvesters. The average grade harvested was 2.35, compared to a hand-picked grade of 2.10 for the same date. However, the FMC harvester picked a mean grade of 2.27 (dollar value of $155 per ton) compared to 2.43 for the Byron harvester (dollar value of $134 per ton). Since the two machines harvested different areas of the field, we cannot be sure whether the differences are due to the machine efficiency or to differences in the maturity of the crop in the different areas. However, the fact that the FMC picked 9.2 percent grade 1 fruit, while the Byron picked only 3.7 percent grade 1, leads us to speculate that the difference lies in harvester efficiency at recovery of small fruit. We were unable to calculate dollar value per acre since the harvested areas for each machine were not reported.

It is obvious from comparing the machine-harvest and hand-picked harvests (Table 2) of the same varieties that machine-harvest led to higher mean grade for all varieties except the SunSeeds line, perhaps due to poorer recovery of small fruit. The average return per acre varied from $729 for 'Duke' to $953 for the SunSeeds line (Table 1). Yields were generally comparable to those in 1992 and less than those of 1991.

Hand-Picked Variety Trial, 1993

We simulated a machine harvest three times over a five-day period (Table 2). 'Duke' was first to reach a mean grade of 2.5 in both the hand-picked and mechanically-harvested plots. 'Napoleon' and 'Flurry-M' were the next varieties to mature, while the SunSeeds line and 'Quest' were the last to mature. Given the better than 0.2 change in grade per day, it may be useful to lump 'Duke', Flurry-M', and 'Napoleon' together as the early maturing varieties, with 'Lafayette', 'Quest', and Sun 3539 about one day later. With unusually hot weather at maturity, there was certainly no more than a 48-hour spread between the earliest and latest varieties. 'Napoleon', and to a lesser extent 'Sun 3539' and 'Flurry-M', were slower to increase in size grade over the harvest period than were the other varieties. This is inconsistent with 1992 when 'Lafayette' was by far the slowest to change size grade. The greatest return per acre, at the harvest nearest optimal maturity, was $1347 for the Sun line and $1346 for 'Quest', but 'Flurry-M' and 'Lafayette' also grossed in excess of $1000 per acre. 'Quest' and 'Lafayette' also performed well in the 1992 hand-picked trial.

The apparent decrease in grade for several varieties between the 9 and 10 August harvests is an artifact. The latter harvest represented only one replicate in the generally weaker and less mature west end of the field. The high percentage of nubs and crooks in this harvest also indicates weakness in this area, perhaps due to low nitrogen status.

Commercial (Machine-Harvested) Variety Trial, 1994

Stands of all varieties except 'Calypso' and 'Bradley' neared or exceeded target population and were greater than the last two years (Table 3). We experienced planter problems with the 'Calypso' pass. 'Bradley' emerged two days later than other varieties and had low seedling vigor. At the time stand counts were taken, not all 'Bradley' seed may have germinated. The lower-than-average yield for 'Bradley' may reflect the fact that it was harvested at low mean size grade, rather than any deficiency in stand.

It is unfortunate that the harvester could not be scheduled into the variety trial on more than one day. It is apparent that 'Bradley,' 'Flurry,' and the Harris-Moran line were harvested a day early. One goal in 1994 was to obtain a high percentage of size grade #3 fruit, suitable for making spears. The entire harvest was probably about a day early for this goal. But with an average size grade for the 10 varieties of 2.44, the cucumbers harvested were of excellent quality.

In comparing the machine- and hand-picked harvests (Tables 3 and 4) of the same varieties, machine-harvest produced a lower mean size grade for four varieties, essentially the same size grade for four varieties, and a greater mean grade for only 'Calypso' and 'Neptune,' indicating excellent recovery of small fruit. In contrast, in 1993 the FMC machine harvested a higher mean size grade than did hand-picking.

The average return per acre varied from $854 for 'Calypso' to $1859 for 'Neptune.' Yields were generally a little less than those in 1991, at about the same maturity, and averaged 2 to 3 tons/acre greater than in 1992 or 1993 (mean across varieties of 10.6 tons/acre in 1994, 12.4 in 1991, 8.0 in 1992, and 7.7 in 1993). The only variety in all four trials was 'Flurry-M'. In 1991 it yielded 11.8 tons/acre, in 1992 only 3.1 tons/acre at similar mean grade. The 1993 yield was 7.1 tons/acre, but the fruit was larger than in 1992. In 1994, at mean grade of only 2.36, 'Flurry' yielded 9.9 tons/acre.

The yields from the hand-picked plots were consistent with this trend. Average hand-picked yield for all varieties declined from 15.1 tons/acre in 1991 to 9.2 tons/acre in 1992 and 8.4 tons/acre when picked at nearly optimal size grade in 1993. Mean hand-picked yield in 1994 rebounded to 11.0 tons/acre, at maturity of 2.5 or less. Hand-picked 'Flurry-M' declined from 8.6 tons/acre in 1991 to 5.7 tons/acre in 1992, but increased to 9.4 tons/acre in 1993 and 9.1 tons/acre in 1994. In addition to weather, other factors may have contributed to the yield differences among years. Stands were greater in 1991 and 1994 than in the other years. The plant population in 1992 and 1993 was lower than the target of 80,000/acre. Specifically for 'Flurry-M', the population was estimated at 65,300/acre in 1991, 55,900 in 1992, 62,000 in 1993, and 94,000 in 1994.

The amount of reject material in loads delivered from the test plots was very low in both years, indicating good weed control and excellent harvester efficiency at expelling dirt and vines. Exceptions were 'Flurry,' which was harvested after dark and in a rush to finish up, and 'Bradley,' a small-vined variety.

Hand-Picked Variety Trial, 1994

The ten varieties were hand-harvested three times over a five-day period (Table 4). 'Atlantis,' 'Discover,' 'Excell,' and 'Lafayette' reached a mean grade of 2.5 by the final hand-harvest, taken only three hours before machine-harvest commenced. With unusually hot weather at maturity, there was certainly no more than a 48-hour spread between the earliest and latest varieties. There were again considerable differences among varieties in the rate of increase in size grade over the harvest period (Figure 1). 'Atlantis' and 'Bradley' increased more than 0.26 mean grade/day, contrasted with only 0.157 for 'HMX 1463.' The varieties 'Flurry' and 'Lafayette' have been grown in each of the last three years. 'Lafayette' increased more rapidly in mean grade than did Flurry in 1993 and 1994. The rate of change was greater in 1994 for both varieties, attributable to the extremely high temperatures at harvest. However, relative rate of increase in grade may not be consistent over all seasons. In 1992, 'Lafayette' was considerably slower to size than was 'Flurry.' Several years of experience and correlation with environmental variables would be necessary to determine whether varietal differences in rate of fruit size increase are predictable.

The greatest return per acre, at the harvest nearest optimal maturity, was $1739 for 'Excell', $1657 for 'Lafayette', and $1656 for Neptune. All varieties exceeded $1100 per acre in the hand-picked trial.

It is apparent when comparing the percentage of nubs and crooks recorded for the last hand-picking with the percentages recorded at the receiving station for the machine-picked fruit, that either the harvester expels many nubs and crooks, or we grade to a higher standard than do the receiving station personnel. Our percentage of nubs and crooks was, on the average, about three times what the receiving station recorded. Nevertheless, most results are consistent between our rating of hand-picked fruit and the grading at the receiving station. Both found 'Flurry' and 'FMX 4841' to have a greater than average nubs plus crooks percentage and 'Bradley' and 'HMX 1463' to have a very low percentage.

Since all hand-harvests were near or below optimal maturity, we were not able to distinguish among varieties in the rate at which they lose dollar value per acre when they pass optimal maturity. All varieties except 'Discover' continued to increase in dollar return per acre between the second and third harvests.

Modeling Cucumber Development in Response to Temperatures

We previously concluded that the number of accumulated heat units (North Carolina State-Washington State model; sum of Tmax-15.5 oC with a 32 oC cutoff) required to reach maturity for a given variety increased with later plantings, indicating that maturity is not a linear function of heat unit accumulation in the Willamette Valley. Results in 1991 through 1994 were consistent with this conclusion. In 1991, it took only 583 AHU to surpass optimal maturity for the variety 'Calypso' with a 5 June planting date. However, in 1990, 690 AHU were required for 'Calypso' to reach a mean grade of 2.5 from a 3 July planting date. In 1994, with a 16 May planting date, only 552 AHU were required to bring 'Calypso' to a mean grade of 2.57 (machine-harvest).

From 1991 through 1994 data on 'Flurry-M', it can also be concluded that the model is not consistent between seasons with different weather patterns. In 1991, with a relatively cool, wet June, it took about 540 AHU to mature 'Flurry-M'. In 1992, however, with unusually high temperatures in June, it took 600. In 1993, with an unusually cool and wet June and July, but with very hot weather near harvest, 'Flurry-M' needed only 501 heat units to reach optimal maturity. In 1994, temperatures were near long-term averages from planting until just a few days before harvest. 'Flurry-M' had not reached a mean grade of 2.5 with 552 AHU. Apparently, vine development, flowering, and fruit development in 1993 occurred normally, despite the very cool weather, and the warmer weather in 1994 did not advance maturity.

It is now apparent that, even with the high temperature cutoff of 32 oC, the North Carolina State-Washington State model gives too much weight to above-normal temperatures at the pre-bloom stage of plant development. Furthermore, the model cannot anticipate any delay in pollination and fruit set that may result from inadequate bee activity at first bloom. It is also our belief that the base temperature of 15.5 oC used in the model is too high. Use of a 10 oC base, and the mean rather than the maximum temperature, along with a penalty for cold weather, may provide a superior model of plant response to temperature.

Weed Control Trial

The high rate of Command, 0.25 lb active/acre, applied pre-emergence produced yields significantly greater than most other treatments and was the only treatment to outyield the hand-weeded control (Table 5). Even at the lower rate, pre-emergence Command was an excellent treatment. Pre-plant incorporation of Command resulted in significantly lower yields and reduced weed control compared to the same rates of Command applied pre-emergence. The high rate of Command, pre-emergence, reduced stands slightly compared to most other treatments in both trials, but yield was not adversely affected. The combination of Command with either Prefar or Alanap did not improve weed control or yield compared to Command alone. The combination of Curbit and Alanap was superior to Curbit alone for both weed control and yield. Registration of Command would provide a valuable tool for growers, particularly in view of its low cost.

Table 1. Nalley's-OSU pickle variety trial, 1993 machine-harvest results      
Variety       %1s   %2s   %3s   %4s   %N&C  %Rej  Tot lb. Grade  $/T  $/A  T/A
Duke          2.7z 13.4  55.6  19.7    7.8   0.8   20400   3.01   85  729  8.6
Flurry-M      3.0  28.1  55.5   8.3    4.7   0.5   17160   2.73  110  775  7.1
Lafayette     4.8  33.2  53.8   3.2    4.2   0.8   18130   2.58  123  949  7.7
Napoleon      6.4  20.8  47.6  22.2    2.5   0.6   19140   2.88  101  813  8.1
Quest         2.4  27.4  62.8   2.7    4.7   0.2   17800   2.70  113  849  7.5
Sunex 3539    3.7  44.8  46.7   1.8    2.8   0.1   17040   2.48  133  953  7.2
zSize categories are as follows:  1) 0.5-1.0 inch diameter, 2) 1.0-1.5 inch,
3) 1.5-2.0 inch, 4) over 2.0 inch.  N&C=nubs plus crooks.  Rej=reject material, 
mainly dirt clods, stems, and roots.  Grade is the weighted average of the 
percentage in each size category, excluding N&C.


Table 2. Nalley's-OSU pickling cucumber variety trial, 1993 hand-picked
harvest results                                                               
Variety    Harvest date  %1s   %2s   %3s   %4s  %N&C  Grade T/acre $/T  $/acre 
Duke          Aug.  6z  18.7  54.7  19.6   1.0   6.0   2.03  3.9   185   715
              Aug.  9    6.4  21.8  48.0  16.7   7.0   2.81  8.3   105   871
              Aug. 10    4.3  18.6  48.2   8.2  20.7   2.72  8.4    98   826
Flurry-M      Aug.  6   15.7  55.3  23.0   1.2   4.8   2.10  4.6   179   818
              Aug.  9    3.7  25.6  54.4   8.4   7.9   2.73  9.4   108  1020
              Aug. 10    3.2  29.1  45.1   9.0  13.5   2.69  9.7   107  1039
Lafayette     Aug.  6   26.2  65.9   5.2   0.0   2.7   1.79  3.8   213   798
              Aug.  9    4.7  39.9  44.4   3.6   7.4   2.51  9.2   128  1175
              Aug. 10    9.8  30.2  47.4   3.5   9.1   2.49  6.7   132   879
Napoleon      Aug.  6   12.3  55.1  27.2   3.6   1.7   2.23  3.7   176   648
              Aug.  9    4.6  21.8  61.7   5.8   6.1   2.73  7.5   111   833
              Aug. 10    8.7  20.6  56.4   7.4   7.0   2.67  5.7   118   677
Quest         Aug.  6   26.1  64.1   6.4   0.0   3.4   1.80  3.3   214   706
              Aug.  9    4.7  37.3  51.2   1.6   5.2   2.52  9.3   128  1188
              Aug. 10    3.8  39.4  47.0   0.8   9.1   2.49 10.6   126  1346
Sunex 3539    Aug.  6   27.8  51.1  18.0   0.0   3.1   1.90  3.7   212   785
              Aug.  9    5.2  43.5  44.3   4.2   2.8   2.49  8.5   134  1134
              Aug. 10    3.5  42.9  45.7   2.4   5.5   2.50 10.4   129  1347  
zHeat units accumulated for each date: Aug. 6, 457; Aug 9, 501; Aug. 10, 510.  
Accumulated heat units = Sum (Tmax-15.5 oC) with a 32 oC cutoff.


Table 3. Nalley's-OSU pickling cucumber variety trial, 1994 machine-harvest results. 
Variety  Plants/A  %1s   %2s   %3s   %4s  %N&C %Rej  lb.  Acres Grade  $/T  $/A  T/A
Atlantis  101,000  5.1  40.3  49.2   0.8   3.4  1.1  8280  0.30  2.48  133 1859 14.0 
Bradley    52,000  6.9  55.1  32.3   1.7   1.4  2.6  8560  0.51  2.30  146 1222  8.4
Calypso    65,000  4.5  36.3  50.3   4.0   4.0  0.9  8220  0.60  2.57  126  854  6.8
Discover  125,000  5.3  40.5  47.7   1.4   4.0  1.1 21425  1.16  2.48  133 1227  9.3
Excell     99,000  4.5  38.8  49.5   2.1   3.9  1.2 28560  1.16  2.52  129 1588 12.3
Flurry-M   94,000  6.5  43.8  37.3   0.6   7.6  4.2  2895  0.15  2.36  133 1319  9.9
FMX 4841   80,000  5.6  44.1  43.1   1.5   4.2  1.5  6815  0.29  2.43  135 1576 11.7
HMX 1463   72,000  6.1  59.7  30.9   0.2   1.7  1.4 13165  0.61  2.26  149 1609 10.8
Lafayette  83,000  5.8  44.8  44.4   0.8   3.3  0.8 26240  1.21  2.42  138 1489 10.8
Neptune   103,000  7.9  42.3  43.4   1.9   2.5  2.1  8840  0.37  2.41  140 1678 12.0


Table 4. Nalley's-OSU pickling cucumber variety trial, 1994 hand-picked harvest
Variety   Harvest date   %1s   %2s   %3s   %4s  %N&C Grade T/acre  $/T $/acre  
Atlantis      July 18z  51.5  41.0   0.3   0.0   5.0  1.48   3.2   249   794
                   21   10.7  52.7  22.3   0.0  14.2  2.14   8.4   150  1255
                   22    5.4  33.0  46.0   3.3  12.3  2.54  11.8   122  1438
Bradley       July 18   80.0  20.0   0.0   0.0   0.0  1.20   1.7   309   526
                   21   16.2  63.9   9.0   0.0  10.8  1.92   7.1   173  1230
                   22    6.8  47.0  35.3   0.0  10.9  2.32  10.5   137  1445
Calypso       July 18   59.7  30.7   3.9   0.0   5.7  1.41   1.9   262   495
                   21    9.1  44.4  32.3   0.0  14.2  2.27   6.9   140   975
                   22    6.5  40.7  44.7   0.0   8.1  2.42   8.6   136  1170
Discover      July 18   40.6  50.3   0.0   0.0   9.1  1.55   3.0   225   667
                   21   10.2  50.3  28.2   1.5   9.8  2.23   9.1   148  1353
                   22    5.0  28.7  45.6   2.2  18.5  2.55  11.8   115  1353
Excell        July 18   30.5  53.0   5.3   0.0  11.2  1.72   4.8   200   964
                   21    7.1  43.3  37.6   1.4  10.6  2.37  10.2   136  1388
                   22    4.4  27.6  56.5   3.2   8.3  2.64  14.7   118  1739
Flurry-M      July 18   49.4  37.8   3.6   0.0   9.3  1.50   2.5   239   602
                   21   10.7  42.6  26.8   2.3  17.6  2.25   6.2   138   856
                   22    8.0  37.3  35.4   1.5  17.8  2.37   9.1   129  1175
FMX 4841      July 18   34.6  43.0   4.2   0.0  18.2  1.63   3.4   199   688
                   21    4.4  50.3  21.1   0.0  24.1  2.22   7.5   126   941
                   22    3.3  43.5  37.7   1.8  13.7  2.44   9.0   124  1116
HMX 1463      July 18   46.2  42.3   8.2   0.0   3.3  1.61   3.5   238   837
                   21    7.9  70.6  16.4   4.0   1.1  2.17   9.0   159  1427
                   22    8.3  57.0  31.2   0.0   3.5  2.24   9.7   153  1483
Lafayette     July 18   31.9  55.2   2.3   0.0  10.5  1.67   2.9   206   608
                   21    7.6  48.2  35.3   0.0   8.9  2.30   8.1   142  1151
                   22    3.7  30.2  52.7   3.2  10.2  2.62  14.1   117  1657
Neptune       July 18   54.0  39.7   0.0   0.0   6.3  1.42   2.8   253   709
                   21   15.4  48.7  21.7   0.0  14.2  2.07   7.1   159  1130
              July 22   11.1  43.5  38.1   0.0   7.3  2.29  11.1   149  1656   
zHeat units accumulated for each date were: July 18, 489; July 21, 536; 
July 22, 552. Accumulated heat units = Sum (Tmax-15.5 oC) with a 32 oC cutoff.


Table 5. 1994 pickling cucumber herbicide trial                                       
Treatment   Rate, lb   Yield   Stand count/  Weed density  Weed controlx  Acre cost of
             ai/acre  kg/plot   3 row feet   per sq. ft.       rating       chemicals  
Command PREz  0.125    16.5         34	        6.5	         8.5	      $2.84

Command PRE   0.25     19.8         26	        4.5	         9.4	      $5.68

Command PPIy  0.125     9.0         33	       19.3	         4.3	      $2.84

Command PPI   0.25     14.8         33	       13.0	         5.7          $5.68

Command PPI   0.125    13.8         34	       39.8	         3.6	     $64.14
Prefar        6.0

Command PPI   0.125    13.5         32	        8.5	         7.0	     $56.94
Alanap        4.0

Curbit  PRE   1.5      11.8         29          9.5	         5.7	     $30.58

Curbit  PRE   1.5      15.3         33	        4.0	         9.0	     $84.68
Alanap        4.0

Alanap  PPI   4.0      17.4         33          9.3	         7.2        $115.40
Prefar        6.0

Handweeded    ---      13.7         32         21.3	         1.2	      ----

Untreated     ---       3.6         34	       23.0	         1.2	      ----

           LSD 0.05     4.6          4         14.7                                    
zPRE:  pre-emergence, incorporated with water.
yPPI:  pre-plant incorporated with rotary tillage.
x0=no control, 10=complete control.

Rate, Timing of Application, Source, and Placement of Nitrogen Fertilizer on Yield of Cauliflower

Cooperator: John Hart, Dept. of Crop and Soil Science

Introduction

Vegetable growers in the Willamette Valley use high rates of nitrogen fertilizers, often exceeding 300 pounds actual N/acre per season. While growers claim that these rates are necessary to achieve maximum yields and quality, a considerable portion of the applied fertilizer is not taken up by the crop. This has raised concerns that the remaining nitrogen may be contributing to nitrate pollution of groundwater. Improved efficiency of nitrogen management in vegetable crops may be possible if the fertilizer could be applied at the time of maximum crop need and placed for maximum contact with the root system. Sources of nitrogen may differ in efficiency of N uptake because of differences in volatility, degree of leaching, or crop preference for ammonium or nitrate.

A trial in 1991 investigated broccoli yield response to a wide range of rates of nitrogen as well as different methods of fertilizer placement. Method of placement did not affect yield. Rates of applied N up to 250 pounds per acre did not significantly increase the soil nitrate or ammonium concentration at the end of the growing season. Cauliflower is planted at lower populations and with greater between-row spacing than is the norm for broccoli. In a 1992 trial cauliflower yield increased with increasing rate of applied nitrogen up to 240 pounds/acre. Yield did not vary with band or broadcast placement of fertilizer at planting but there was a trend toward greater yield with the banded application. Broadcast application of sidedressed nitrogen was superior to banded application. The purpose of these trials was to confirm results obtained in 1992 and to investigate the effects of timing of application of sidedressed nitrogen as well as the form of the sidedressed nitrogen. A second purpose was to study soil nitrogen accumulation as a function of rate of applied nitrogen and presence of a winter catch crop and to monitor loss of nitrate through the soil profile in the winter following the crop.

Methods

'Snowball Y' cauliflower was direct-seeded in a Willamette silt loam, pH 6.0, at the NWREC on 10 June in both 1993 and 1994. Rows were 12 inches apart with a plant density of about two/inch in the row. Plot preparation included a broadcast and incorporated application of 10N-8.7P-16.7K fertilizer at 600 pounds/acre, boron at 2.0 pounds/acre, ammonium molybdate at 2 ounces/acre, trifluralin at 0.75 pounds/acre, and chlorpyrifos at 1.3 pounds/acre. On 27 July, 1993 (20 July, 1994) the seedlings were lifted with a shovel and transplanted bare-root into their final location. Soil preparation was the same as for the seed bed. Transplants were set in rows 2.5 feet apart with 18 inches between plants in the row. Plot size was three rows, 20 feet long. All three rows were harvested.

The initial application of 40 pounds N/acre was either broadcast as urea or banded as calcium-ammonium nitrate solution (CAN-17) three inches to the side of the row immediately after transplanting and irrigated in. The remaining nitrogen was broadcast or banded on the appropriate plots on 2 September or on 2 and 21 September, 1993 (24 August or 24 August and 21 September, 1994) depending on the treatment (Table 6). Sidedress N source was either urea, calcium nitrate (CN), or CAN-17 as appropriate for the treatment. Treatments were in randomized complete block design with four replications. The plots were sprinkler-irrigated as necessary and harvested on 8 and 19 October, and 2 November, 1993 (10 and 21 October, 1994). Two sets of plots were overseeded with 'Wheeler' cereal rye on 7 September to determine the feasibility of overseeding in cauliflower as a means of establishing a winter nitrogen catch crop. Following the last harvest in 1993, soil in treatments 1, 2, 3, 4, 5, and 11 was sampled to 48-inch depth in 12-inch increments. The plots were maintained through the winter in order to resample for soil nitrogen content and cover crop biomass accumulation. Shoots were clipped to 1 inch above the soil surface on 18 April. After weighing, subsamples were weighed, dried, reweighed, and submitted for analysis of total N. The soil of treatments 1-5 and 10 were sampled in 1-foot increments to 5-foot depth on 21 April. Samples were frozen and submitted for analysis of nitrate and ammonium content.

Results and Discussion

For the plots receiving only broadcast applications of urea, yield and mean head weight increased with increasing rate of nitrogen to a maximum at 240 pounds N/acre in 1993 and 180 pounds in 1994 (Table 7). The same trend was not true for the percentage of Grade No. 1 heads (defect-free), as only an increase from no applied nitrogen to the lowest rate of 60 pounds/acre caused an increase in quality in 1993 and N rate did not affect curd quality in 1994. In 1992, quality continued to increase with increasing increments of applied N. Previous work at the OSU vegetable farm suggested that the optimum rate of nitrogen application to cauliflower is in the range of 150 to 200 pounds/acre, similar to the results obtained in these trials.

Banded versus broadcast application of N at planting had no significant effect on yield or quality in 1993 (Table 8). In 1992, there was a greater trend toward higher yield and head size with a banded application but it was also not a statistically significant effect. Apparently, even with rows 30 inches apart, enough feeder roots establish in the soil between the rows that concentrating the fertilizer near the plant row is not a great advantage. This is in agreement with results obtained on broccoli grown on 16 or 20-inch row spacing.

Banded versus broadcast application of the sidedressed nitrogen also did not result in significant differences (Table 8). This is in contrast to 1992, when greater yield and mean head size occurred with a broadcast application. However, the single greatest yield in this trial was with the combination of broadcast fertilizer at planting, broadcast sidedress fertilizer, and a rate of 240 pounds N/acre.

Overseeding cereal rye about four weeks (1993) or seven weeks (1994) before first harvest did not reduce cauliflower yield (Table 9). This is consistent with results obtained in 1992, even though the rye development was greater than in 1993 and 1994 than in 1992.

Use of calcium nitrate, rather than urea, as the source for the sidedressed nitrogen had no effect on yield for the sum of the harvests in either year (Tables 10 and 11). Calcium-ammonium nitrate, as compared to urea, also had no effect on yield or head size in 1994 (Table 12).

Splitting the sidedressed N application such that half was not applied until eight or nine weeks after transplanting had no effect on yield or quality (Tables 11 and 13). However, there was a significant interaction of N source x timing of application affecting mean head weight and gross yield in 1994 (Table 11): splitting the sidedress N application decreased yield with urea as N source, but increased it with calcium nitrate. Further testing would be needed to determine if this effect is reproducible and biologically significant. Number of heads harvested at either harvest was not affected by N source or splitting the sidedress N application, indicating these factors did not affect maturity (data not shown).

In 1993, cauliflower effectively depleted the root zone of nitrate and ammonium at all rates of applied N except 240 pounds/acre (Table 14). Even at the high rate of N, residual soil nitrate was comparable to that before any fertilizer was applied. Apparently, cauliflower is similar to broccoli in N uptake efficiency, even though it was planted at a lower plant population and wider between-row spacing. Calcium nitrate versus urea as N source had no effect on residual soil nitrate and ammonium levels at harvest (Table 15). Splitting the sidedressed N also did not affect residual soil N concentrations (Table 16).

Cereal rye growing on plots that had been fertilized with 120 or 240 pounds N/acre accumulated a shoot dry weight of 0.55 and 1.19 tons/acre, respectively, on 18 April. This corresponds to nitrogen uptake of 16 and 36 pounds N/acre, respectively. Considering that only about 45 pounds of mineral N was present in the top foot of soil immediately after harvest of plots fertilized with 240 pounds N/acre, uptake of 36 pounds N/acre appears to represent recovery of a significant portion of the residual mineral N. Overseeding a grain into cauliflower may be a practical alternative for establishing an effective N catch crop in this late-harvested vegetable crop.

When re-sampled in the spring of 1994, after 24 inches of precipitation, rate of applied N had no effect on soil ammonium concentration (Table 17). Soil nitrate concentration tended to increase with greater rates of applied N. The nitrate concentration of the surface 10 inches of soil decreased from those immediately after harvest, while that of the next 30 inches of the profile increased, indicating probably of movement of nitrate with the winter rainfall. The rye cover crop significantly reduced soil nitrate concentration at all but the 20-30 inch depth, but had no effect on soil ammonium concentration.

Table 6. List of treatments, cauliflower N utilization trials, NWREC            
No.  Total N   Placement             Placement and timing       
     applied   at planting              of sidedress                              
-------------------------------lb/A--------------------------------------------- 
1993
 1      0      0                    None
 2     60     40 broadcast, urea    20 broadcast, 5 weeks
 3    120     40 broadcast, urea    80 broadcast, 5 weeks
 4    180     40 broadcast, urea   140 broadcast, 5 weeks
 5    240     40 broadcast, urea   200 broadcast, 5 weeks
 6    120     40 banded, urea       80 broadcast, 5 weeks
 7    120     40 broadcast, urea    80 banded, 5 weeks
 8    120     40 banded, urea       80 banded, 5 weeks
 9    120     40 broadcast, urea    80 broadcast, 5 weeks; overseed, 6 weeks
10    240     40 broadcast, urea   200 broadcast, 5 weeks; overseed, 6 weeks
11    120     40 broadcast, urea    80 broadcast as calcium nitrate, 5 weeks
12    240     40 broadcast, urea   200 broadcast as calcium nitrate, 5 weeks
13    120     40 broadcast, urea    40 broadcast, 5 weeks;  40 broadcast, 9 weeks
14    240     40 broadcast, urea   100 broadcast, 5 weeks; 100 broadcast, 9 weeks

1994
 1      0      0                     0
 2     60     40 broadcast, urea    20 broadcast, 5 weeks, urea
 3    120     40 broadcast, urea    80 broadcast, 5 weeks, urea
 4    180     40 broadcast, urea   140 broadcast, 5 weeks, urea
 5    240     40 broadcast, urea   200 broadcast, 5 weeks, urea
 6    120     40 broadcast, urea    80 broadcast, overseed, urea
 7    240     40 broadcast, urea   200 broadcast, overseed, urea
 8    120     40 broadcast, urea    80 broadcast, calcium nitrate
 9    180     40 broadcast, urea   140 broadcast, calcium nitrate
10    120     40 broadcast, urea    40 bcast, 5 weeks; 40 bcast 9 weeks, CN
11    180     40 broadcast, urea    70 bcast, 5 weeks; 70 bcast 9 weeks, CN
12    120     40 broadcast, urea    40 bcast, 5 weeks; 40 bcast 9 weeks, urea
13    180     40 broadcast, urea    70 bcast, 5 weeks; 70 bcast 9 weeks, urea
14    180     40 banded, CAN-17    140 banded, 5 weeks, CAN-17                    


Table 7. Effect of rate of broadcast urea nitrogen on yield, 
head size, and quality of cauliflower, NWREC                 
N rate         Mean head       Grade No. 1     Total yield
(lb/acre)       wt. (g)         heads (%)      (tons/acre)   
1993
  0               565              39.5            7.1          
 60               664              62.5            8.5          
120               809              62.7           10.6          
180               978              58.6           11.9          
240              1002              53.9           12.4
      LSD (0.05)  170              16.6            2.6       
1994
  0               580              41.1            6.3        
 60               740              60.2            8.2        
120               856              56.7           10.1       
180              1046              66.5           12.3       
240              1043              61.2           12.1
      LSD (0.05)  162               NS             2.2           


Table 8. Effect of broadcast versus banded application of initial and 
sidedressed nitrogen on yield, head size and quality of cauliflower, 1993
Placement    Placement          Mean head   Grade No. 1   Total yield
at planting  at sidedress        wt. (g)     heads (%)    (tons/acre)    
Broadcast    Broadcast             809          62.7         10.6
             Banded                924          60.2         11.3
Banded       Broadcast             923          51.5         10.9
             Banded                889          63.0         11.0
      Broadcast at planting mean   867          61.4         11.0
      Banded at planting mean      906          57.8         11.0
          Significance, planting    NS           NS           NS
      Broadcast at sidedress mean  866          57.1         10.8
      Banded at sidedress mean     906          61.6         11.2
          Significance, sidedress   NS           NS           NS        


Table 9. Effect of overseeding cereal rye on cauliflower yield, 
head size, and quality at two rates of nitrogen, NWREC          
Treatment       N rate    Mean head   Grade No. 1    Total yield
               (lb/acre)   wt. (g)     heads (%)     (tons/acre)
1993
Overseeded       120         859         66.3          10.7
                 240         988         54.5          11.2
                      Mean   924         60.4          11.0
Not overseeded   120         809         62.7          10.6
                 240        1002         53.9          12.4
                      Mean   906         58.3          11.5
               Significance   NS          NS             NS
1994
Overseeded       120         898         60.7          10.4
                 240        1025         60.0          11.6
                      Mean   962         60.4          11.0
Not overseeded   120         856         56.7          10.1
                 240        1043         61.2          12.1
                      Mean   950         59.0          11.1
               Significance   NS          NS             NS     


Table 10. Effect of sidedressed nitrogen source on cauliflower yield,
head size, and quality at two rates of nitrogen, NWREC, 1993          
N source        N rate    Mean head   Grade No. 1   Total yield
               (lb/acre)   wt. (g)     heads (%)    (tons/acre)     
Urea             120         809         62.7          10.6    
                 240        1002         53.9          12.4
                       Mean  906         58.3          11.5
Calcium nitrate  120         857         67.7          10.2
                 240         972         60.2          12.2
                       Mean  915         63.9          11.2
                Significance  NS          NS            NS          


Table 11. Effect of sidedressed nitrogen source and timing on cauliflower 
yield, head size, and quality at two rates of nitrogen, NWREC, 1994        
N source        Timing   N rate    Mean head   Grade No. 1   Total yield
                        (lb/acre)   wt. (g)     heads (%)    (tons/acre)
Urea             early   120         856         56.7          10.1   
                         180        1045         66.5          12.3
                        Mean, early  951         61.6          11.2

                 late    120         783         61.9           9.1
                         180         951         62.0          10.9
                        Mean, late   867         61.9          10.0
                        Mean, urea   909         60.0          10.6

Calcium nitrate  early   120         849         61.5           9.6
                         180         966         64.2          11.1
                        Mean, early  908         62.9          10.4

                 late    120         904         65.5          10.4
                         180        1092         55.1          13.4
                        Mean, late   998         60.3          11.9
                        Mean, CN     953         61.6          11.1
    Significance, N rate             **           NS            **
                  N source           NS           NS            NS
                  Timing             NS           NS            NS
                  Source x Timing    **           NS            **
                  Other interactions NS           NS            NS      


Table 12. Effect of urea versus CAN-17 as N source on cauliflower, 
head size, and quality at 180 pounds N/acre, NWREC, 1994         
N source            Mean head    Grade No. 1    Total yield
                     wt. (g)      heads (%)     (tons/acre)      
Urea                  1045          66.5           12.3
CAN-17                1063          61.4           12.3
          Significance NS            NS             NS           


Table 13. Effect of splitting the application of sidedressed nitrogen on
cauliflower, head size, and quality at two rates of nitrogen, NWREC, 1993  
Timing of Application    N rate     Mean head    Grade No. 1    Total yield
                        (lb/acre)    wt. (g)      heads (%)     (tons/acre)
All at 5 weeks             120         809          62.7           10.6
                           240        1002          53.9           12.4
                                 Mean  906          58.3           11.5

Half at 5 weeks,           120         966          61.5           11.8
remainder at 8 weeks       240         928          67.5           11.8
                                 Mean  947          63.5           11.8
                          Significance  NS           NS             NS      


Table 14. Effect of rate of broadcast nitrogen on soil nitrate and ammonium
concentrations (ppm) following final cauliflower harvest, 11 November, 1993 
                                          N rate, lb/A                        
                                       0    60   120   160    240  LSD(.05)
Depth of sample (inches)                                                    
                          Pre-plant  -----------Post-harvest------------   
Nitrate
 0-10                      10.9      0.3   0.6   0.6   5.8   10.8    7.9   
10-20                       5.2      0.6   0.4   0.3   1.3    1.4    NSD   
20-30                       3.3      1.3   0.5   0.6   1.0    1.2    NSD   
30-40                       2.3      1.4   1.2   1.1   0.8    1.3    NSD
Ammonium
 0-10                       5.1      2.0   2.3   2.5   3.6    5.3    2.0
10-20                       4.9      1.3   1.7   1.8   2.4    2.2    NSD
20-30                       3.8      1.7   1.8   2.0   1.8    1.8    NSD
30-40                       3.5      1.7   1.6   1.8   1.5    1.5    NSD   


Table 15. Effect of nitrogen source and rate on soil nitrate and ammonium 
concentrations (ppm) following final cauliflower harvest, 11 November, 1993     
                               Urea               Calcium nitrate
Depth of sample (inches)               N rate, lb/A              
                             120    240   Mean    120   240  Mean  LSD (0.05)z
Nitrate
 0-10                        0.6   10.8   5.7     0.6   7.9   4.3    7.9
10-20                        0.3    1.4   0.8     0.4   1.4   0.9    NSD
20-30                        0.6    1.2   0.9     0.9   1.2   1.0    NSD
30-40                        1.1    1.3   1.2     1.0   1.3   1.2    NSD
Ammonium
 0-10                        2.5    5.3   3.9     2.4   3.6   3.0    2.0
10-20                        1.8    2.2   2.0     1.6   1.9   1.8    NSD
20-30                        2.0    1.8   1.9     1.6   1.8   1.7    NSD
30-40                        1.8    1.5   1.7     1.5   1.6   1.6    NSD    
zLSD for N source x N rate interaction.  Main effect of N source 
 nonsignificant for both ammonium and nitrate at all depths.


Table 16. Effect of splitting the sidedress urea application and nitrogen rate
on soil nitrate and ammonium concentrations (ppm) following final cauliflower 
harvest, NWREC, 11 November, 1993                                              
                             Single sidedress       Split sidedress
                                           N rate, lb/A                          
Depth of sample (inches)     120    240  Mean       120   240  Mean  LSD (0.05)z
Nitrate
 0-10                        0.6   10.8   5.7       0.8   6.0   3.4    7.9
10-20                        0.3    1.4   0.8       0.4   1.6   1.0    NSD
20-30                        0.6    1.2   0.9       0.9   1.6   1.2    NSD
30-40                        1.1    1.3   1.2       1.2   1.3   1.2    NSD
Ammonium
 0-10                        2.5    5.3   3.9       2.2   6.1   4.1    2.0
10-20                        1.8    2.2   2.0       2.0   1.9   2.0    NSD
20-30                        2.0    1.8   1.9       1.9   1.9   1.9    NSD
30-40                        1.8    1.5   1.7       1.6   1.7   1.6    NSD      
zLSD for sidedress x N rate interaction.  Main effect of splitting the 
sidedress N application nonsignificant for both ammonium and nitrate at all depths.


Table 17. Effect of rate of applied nitrogen and a rye cover crop on 
residual soil nitrate and ammonium concentrations, 28 April, 1994          
Sample depth              Rate of applied urea, lb/acre          LSD (0.05)
(inches)                  0    60   120   180   240  240 (cover)           
                        ------------------------ppm---------------------
Nitrate
 0-10                   0.5   1.1   1.7   0.8   2.6      0.8         1.2
10-20                   0.6   1.2   1.6   2.7   5.0      3.0         0.8
20-30                   0.5   1.1   1.4   4.5   4.4      3.7         2.2
30-40                   0.3   0.6   0.8   2.1   4.0      2.7         1.3
Ammonium
 0-10                   2.4   2.0   2.5   2.1   2.3      2.2          NS
10-20                   2.6   2.1   2.3   1.9   2.0      1.9          NS
20-30                   2.2   2.0   2.0   2.1   1.8      2.0          NS
30-40                   2.0   1.5   1.7   1.7   2.6      1.7          NS   

Effect of Nitrogen Rate, Source, Placement, and Timing on Sweet Corn Yield and Nitrogen Uptake

Cooperator: John Hart, Dept. of Crop and Soil Science

Introduction

The justification for this trial is similar to that for cauliflower, reported earlier. A sweet corn trial in 1992 indicated that source and placement of nitrogen fertilizer had little effect on yield or quality of sweet corn. Rates of nitrogen application greater than 60 pounds per acre resulted in accumulation of significant amounts of nitrate-N in the soil. The purpose of these trials was to confirm 1992 results and to determine if yield of sweet corn would be affected by source or timing of application of nitrogen fertilizer.

Methods

'Jubilee' sweet corn was seeded into a Willamette silt loam, pH 5.9, at the NWREC on 13 May, 1993, and 25 May, 1994. Plot preparation included a broadcast and incorporated application of potassium sulfate at 250 pounds/acre, disking and cultimulching. Triple superphosphate was banded at 130 pounds/acre, two inches to the side and two inches beneath the seed row on all plots. Forty pounds of nitrogen/acre as urea, ammonium nitrate, calcium-ammonium nitrate (CAN-17), or urea-ammonium nitrate (UAN-32) was also shanked in at 2 inches beneath and 2 inches to the side of the seed row on all but the zero nitrogen treatment (Table 18). The prilled urea and ammonium nitrate were applied in the same band as the superphosphate. The liquid CAN-17 and UAN-32 were applied with separate shanks mounted behind the superphosphate shanks.

Plot size was 15 feet wide (six rows) by 30 feet long. Spacing between rows was 30 inches. Immediately after planting atrazine was applied at 2.0 pounds/acre and alachlor at 3.0 pounds/acre. The remaining nitrogen was shanked in (banded) or broadcast to the appropriate plots at planting or on 21 June, 1993 or 29 June, 1994 (split application). Treatments consisting of various rates, sources, and sidedress application methods were in randomized complete block design with four replications.

The plots were sprinkler-irrigated as necessary and harvested on 24 August, 1993 and 2 September, 1994. Following completion of harvest, the stover was mowed and left in place on the plots. The plots were sampled for residual soil nitrate and ammonium concentration on 22 October, 1993, before the onset of fall rains, and their identity was maintained over the winter so that samples could be taken in the spring of 1994.

Results and Discussion

When all the sidedressed nitrogen fertilizer was banded as urea (Treatments 1-5), yield increased with increasing rate of N to a maximum at 180 pounds N/acre in both years (Table 19). However, the yields at 120 and 240 pounds N/acre were not significantly different than at 180 pounds/acre. Mean ear weight, number of ears harvested, ear length, and tipfill also tended to be greatest at 180 or 240 pounds N/acre, but there were no significant differences among the three greatest rates of N. Kernel moisture content was approximately 73 percent for all treatments.

The other combinations of N source and application method were at 120 pounds total N/acre in 1993 and 120 or 180 pounds N/acre in 1994. Comparisons of N utilization are based on banded urea at planting and broadcast urea sidedress, with a split application, as the standard. Mean yield of corn fertilized at 120 pounds N/acre did not vary significantly with nitrogen source in either year (Tables 20 and 21). This is consistent with results obtained in 1992. Past research at NWREC with urea, ammonium nitrate, and other solid nitrogen sources indicated no consistent differences among nitrogen sources in effects on corn yields.

When comparing the effect of the timing of the sidedressed N application, and averaged over urea and ammonium nitrate as N source, a split or delayed application of the sidedressed N appeared slightly superior to applying all fertilizer at planting for yield, number of ears harvested, ear weight, and tipfill, but the differences were not significant in either year (Table 22). An effect of split application might have been expected in 1993 because of the greater than normal precipitation (15.3 cm) and, thus, leaching potential, during the interval between planting and the delayed sidedress application. However, in 1994, precipitation was only 4.5 cm during the interval between planting and the delayed sidedress, indicating little potential for leaching.

In a 1993 comparison of broadcast versus banded application of sidedressed urea or ammonium nitrate fertilizer (Table 23), there were no significant effects on yield or quality. However, the number of ears/acre was greater with broadcast application of the sidedressed nitrogen.

In comparing CAN-17 with urea as N source, at 180 pounds N/acre, CAN-17 again tended to produce greater yield, but the difference was not significant (Table 24).

Sweet corn production with zero to 120 pounds applied N/acre effectively reduced nitrate concentration in the first 40 inches of the soil profile during the growing season of 1993 (Table 25). However, at 180 or more pounds N/acre, soil nitrate concentrations were greatly elevated in the surface 10 inches of soil. Soil ammonium concentrations were not greatly affected by sweet corn fertilized with any rate of N. These results are fairly consistent with those obtained in 1992, when levels of both nitrate and ammonium were greatly increased in the surface layer of soil by rates as low as 120 lb N/acre. This is in contrast to soil cropped with broccoli: in 1991 and 1992 rates of nitrogen up to 250 pounds/acre did not increase nitrate and ammonium levels beyond those present at planting. There is very little indication in this experiment of movement of applied nitrogen beyond the root zone. Increased nitrate and ammonium levels were generally confined to the surface 10 inches.

Plots were again sampled for nitrate and ammonium content in the spring of 1994, following approximately 24 inches of precipitation (Table 26). Ammonium concentration did not vary with rate of applied N. Soil nitrate concentration again did not vary with applied N from 0 to 120 pounds N/acre. However, at the higher rates of N, nitrate concentration remained elevated, although only at depths greater than 20 inches. This is indirect evidence for the leaching of nitrate from the surface 20 inches of soil toward the vadose zone.

In contrast to 1992 and 1993, rate of applied N had relatively little effect on post-harvest soil concentrations of ammonium and nitrate in 1994 (Table 27). Soil nitrate content tended to be slightly elevated in the surface 12 inches of soil at the optimal N rate of 180 pounds/acre, but the effect was not significant and there was no effect at all at suboptimal rates of N. At the greatest rate of applied N, soil nitrate, but not ammonium levels, were significantly increased, but to a lesser extent than in the previous years. Any effect of N rate at greater depth was masked by the greater-than-normal nitrate concentrations that existed at time of planting. There was a small but significant effect of treatment on soil ammonium concentration at 36 to 48 inches. The form of applied N had no effect on soil nitrate concentrations at harvest, but there was, again, a small effect on soil ammonium content at 36 to 48-inch depth.

The high levels of residual fertilizer present at rates of nitrogen needed for acceptable yields is in contrast to the situation for broccoli and is a cause for concern. Apparently sweet corn is less efficient at taking up applied nitrogen than is broccoli. Measurements of the nitrogen content of the above-ground biomass of each crop support this conclusion. This indicates the need for more research on improving nitrogen uptake efficiency in sweet corn.

Table 18. List of N application treatments, sweet corn nitrogen 
utilization trial, NWREC                                                
No. N rate  N source  Banded at    Broadcast at   Sidedress rate, methodz
    (lb/A)          seeding (lb/A) seeding (lb/A)  (lb/A)                
1993
 1      0    None         0            0              0
 2     60    Urea        40            0             20 broadcast
 3    120    Urea        40            0             80 broadcast
 4    180    Urea        40            0            140 broadcast
 5    240    Urea        40            0            200 broadcast
 6    120    NH4NO3      40            0             80 broadcast
 7    120    CAN-17      40            0             80 banded
 8    120    UAN-32      40            0             80 banded
 9    120    Urea        40           80              0       
10    120    Urea        40            0             80 banded
11    120    NH4NO3      40           80              0       
12    120    NH4NO3      40            0             80 banded
13    180    CAN-17      40            0            140 banded
1994
 1      0    None         0             0              0
 2     60    Urea        40             0             20 broadcast
 3    120    Urea        40             0             80 broadcast
 4    180    Urea        40             0            140 broadcast
 5    240    Urea        40             0            200 broadcast
 6    120    NH4NO3      40             0             80 broadcast
 7    120    CAN-17      40             0             80 banded
 8    120    UAN-32      40             0             80 banded
 9    120    Urea        40            80              0       
10    120    NH4NO3      40            80              0       
11    180    Urea        40           140              0       
12    180    NH4NO3      40           140              0       
13    180    NH4NO3      40           None           140 broadcast
14    180    CAN-17      40           None           140 dribble band
15    180    UAN-32      40           None           140 dribble band     
zNitrogen sidedressed on 21 June, 1993 and 29 June, 1994.


Table 19. Effect of rate of urea-nitrogenz on the yield of sweet corn,
NWREC, 1993 and 1994                                                    
N rate        Yield       No. ears   Ear wt.    Ear length   Tipfilly
(lb/A)        (T/A)       per acre    (g)        (inches)            
1993
  0	       2.7	   10680      241	   8.2	       2.9
 60	       5.8         20060      268	   8.7	       2.9
120	       7.5         25400      268	   8.9	       3.1
180	       7.7	   25960      272	   8.8	       3.3
240            7.5         25510      268	   9.0	       3.4
  LSD (0.05)   1.5          6330      NSD          0.3         0.4
1994
  0	       7.2	   37020      198	   8.6	       3.0 
 60	      10.6         37333      263	   9.4	       3.8 
120	      11.6         38990      271          9.3	       3.9 
180	      12.5	   42040      275	   9.5	       4.2 
240	      10.5	   35830      268          9.4	       3.8 
  LSD (0.05)   2.1           NS        50          0.3         0.5    
zForty pounds N/acre banded at planting, remainder broadcast five
 weeks later.
yFive-point scale with 5=perfect fill.


Table 20. Effect of four nitrogen sources, at 120 pounds N/acrez, 
on the yield of sweet corn, NWREC, 1993 and 1994                 
N source    Yield      No. ears    Ear wt.   Ear length   Tipfill
            (T/A)      per acre      (g)      (inches)           
1993
Urea	     7.1	22670        286	 8.9	     3.3
NH4NO3       7.0	23980	     271	 8.9         3.5
CAN-17       7.3	24960        267	 8.9         3.3
UAN-32       6.7        22560        269         8.7         3.2
LSD (0.05)    NS          NS          NS          NS          NS
1994
Urea        11.6	38990	     271	 9.3	     3.9
NH4NO3      11.3	33210	     307         9.5         4.1
CAN-17      11.2	39970        267	 9.4         4.1
UAN-32	    11.1        40030        266         9.2         3.7
LSD (0.05)    NS          NS          NS          NS          NS 
zForty pounds N/acre banded at planting, 80 pounds N/acre banded 
 five weeks later.


Table 21. Effect of four nitrogen sources, at 180 pounds N/acrez, on the 
yield of sweet corn, NWREC, 1994                                         
N source    Yield      No. ears   Ear wt.  Ear length   Tipfill  Moisture
            (T/A)      per acre     (g)     (inches)                (%)  
Urea	    12.5	42040	    275	      9.5	  4.2      71.3
NH4NO3      12.2        39750	    284	      9.3         4.2      71.4
CAN-17      11.7	42250       253	      9.5         3.8      74.2
UAN-32      10.6        37350       256       9.4         4.1      73.6
LSD (0.05)   1.6         NS          NS        NS          NS       NS   
zForty pounds N/acre banded at planting, 140 pounds N/acre banded five 
 weeks later.


Table 22. Interaction of nitrogen source and timing of sidedress nitrogenz
application on the yield of sweet corn, NWREC, 1993 and 1994             
N source   Timing   Yield   No. ears  Ear wt.  Ear length   Tipfill
                    (T/A)   per acre    (g)      (inches)                 
1993
Urea      planting   7.0     24310      265        8.9        3.0
Urea      5 weeks    7.5     25400      268        8.9        3.1
NH4NO3    planting   7.3     24850      269        8.8        3.1
NH4NO3    5 weeks    7.7     26270      271        8.8        3.3
                     NS        NS        NS         NS         NS
1994
Urea      planting  11.2     38880      264        9.3        3.9
Urea      5 weeks   12.0     40510      273        9.4        4.1
NH4NO3    planting  11.2     35070      292        9.4        4.2
NH4NO3    5 weeks   11.8     36480      295        9.4        4.2
LSD (0.05)           NS        NS        21         NS         NS        
zRate of nitrogen = 120 pounds/acre in 1993 with 80 pounds/acre 
 sidedressed.  Mean of nitrogen applications of 120 and 180 
 pounds/acre in 1994.  Sidedress application average of 80 and 
 140 pounds/acre in 1994.


Table 23. Effect of banded versus broadcast sidedress nitrogenz 
application on sweet corn yield, NWREC, 1993                       
N source  Sidedress   Yield  No. ears  Ear wt.  Ear length  Tipfill
          method      (T/A)  per acre    (g)     (inches)          
Urea      Band         7.1    22670      286       8.9       3.3
Urea      Broadcast    7.5    25400      268       8.9       3.1
NH4NO3    Band         7.0    23980      271       8.9       3.5
NH4NO3    Broadcast    7.7    26270      271       8.8       3.3
LSD (0.05)              NS     2950       NS        NS        NS   
zRate of applied nitrogen = 120 pounds/acre.  Sidedressed five 
weeks after planting.


Table 24. Effect of CAN-17 versus ureaz as N source on the 
yield of sweet corn, NWREC, 1993                                  
N source    Yield     No. ears     Ear wt.   Ear length   Tipfill
            (T/A)     per acre       (g)      (inches)           
CAN-17       8.3        28890        260         9.0        3.3
Urea         7.7        25980        272         8.8        3.3
LSD (0.05)    NS          NS          NS          NS         NS  
zNitrogen applied at 180 pounds/acre.


Table 25. Effect of rate of nitrogen on post-harvest soil nitrate and 
ammonium concentrations, 25 October, 1993                                   
Sample depth            Rate of applied urea, lb/acre   LSD (0.05) Pre-plant
(inches)                 0    60     120    180    240                      
                        ----------------------ppm------------------------
Nitrate
 0-10                   2.6   2.9    2.9    7.1   22.2       4.7      3.6
10-20                   1.3   1.0    1.4    2.1    7.0       3.1      2.2
20-30                   1.4   2.2    1.8    2.3    4.0       NS       2.3
30-40                   1.9   1.9    2.4    2.7    3.1       NS       3.1
Ammonium
 0-10                   2.6   2.1    1.9    3.3    2.9       NS       3.1
10-20                   1.9   1.6    1.3    1.8    2.0       NS       3.6
20-30                   1.8   2.0    1.9    2.0    2.1       NS       1.9
30-40                   1.9   1.7    2.2    2.4    1.9       NS       2.3    


Table 26. Effect of rate of nitrogen on residual soil nitrate and 
ammonium concentrations, 28 April, 1994                          
Sample depth            Rate of applied urea, lb/acre   LSD (0.05)
(inches)                 0    60     120    180    240           
                        -----------------ppm--------------------
Nitrate
 0-10                   1.2   0.9    1.4    0.9    1.6       NS
10-20                   1.5   1.5    2.2    2.1    2.9       NS
20-30                   1.7   1.7    2.8    3.9    5.2       1.2
30-40                   1.6   1.6    2.6    4.6    6.1       2.5
Ammonium
 0-10                   3.1   2.7    3.0    3.1    3.3       NS
10-20                   2.6   2.5    2.5    2.3    2.3       NS
20-30                   3.1   2.3    2.6    2.3    2.2       NS
30-40                   2.7   2.1    2.5    2.5    2.4       NS  


Table 27. Effect of rate of nitrogen on post-harvest soil nitrate 
and ammonium concentrations, 9 September, 1994                              
Sample depth            Rate of applied urea, lb/acre   LSD (0.05) Pre-plant
(inches)                 0    60     120    180    240                        
                        ----------------------ppm-------------------------
Nitrate
 0-12                   0.3   0.4    0.8    4.6   10.9       8.6      0.6
12-24                   1.4   3.0    1.5    8.7    7.9       NS       8.2
24-36                   9.2   7.6    3.8    9.7    6.2       NS      14.0
36-48                   8.7   8.7    5.0    7.1    8.0       NS       2.3
Ammonium
 0-12                   2.1   2.2    2.5    2.2    5.4       NS       2.1
12-24                   2.1   2.4    2.8    2.2    5.2       NS       1.8
24-36                   2.5   2.3    2.9    2.3    6.5       NS       1.8
36-48                   2.0   2.2    2.4    1.6    4.0       1.4      1.6   


Table 28. Effect of nitrogen source on post-harvest soil nitrate and 
ammonium concentrations at 180 pounds applied N/acre, 9 September, 1994
                                 N source             
Sample depth (inches)  Urea  NH4NO3  CAN-17  UAN-32  LSD (0.05)
                       ------------------ppm-------------------
Nitrate
 0-12                   4.6    0.4    0.8     4.6       NS
12-24                   8.7    3.0    1.5     8.7       NS
20-36                   9.7    7.6    3.8     9.7       NS
36-48                   7.1    8.7    5.0     7.1       NS
Ammonium
 0-12                   2.2    3.5    3.0     3.8       NS
12-24                   2.2    3.0    3.5     2.6       NS
24-36                   2.3    2.8    2.8     2.8       NS
36-48                   1.6    3.5    2.7     2.5      1.4             


Nitrogen Rate on Yield of Green Beans, Beets, and Carrots, and Residual Mineral Nitrogen Concentration of Willamette Silt Loam Soil

Cooperator: John Hart, Dept. of Crop and Soil Science

Introduction

A survey of grower fields was initiated in 1993, in which 30 fields were sampled for nitrate and ammonium-N concentrations before fertilization and were then cropped to beans, beets, broccoli, carrots, cauliflower, and sweet corn. At the end of the growing season, the fields were tested for residual nitrate and ammonium concentration. This survey was repeated in 1994 on 34 fields. To provide a basis of comparison with the grower fields, crop yield and residual mineral N were measured in NWREC trials as a function of applied N for beans, beets, carrots, cauliflower, and sweet corn. The cauliflower and sweet corn trials involved other factors such as N source and timing of N application and results are presented elsewhere. The bean, beet, and carrot results are presented here.

Methods

All crops were seeded to a Willamette silt loam, pH 6.1, at NWREC. Plot preparation for all crops included a broadcast and incorporated application of potassium sulfate at 250 pounds per acre, disking and cultimulching. Pre-plant soil samples were obtained to four-foot depth, in one-foot increments, on 21 April. The samples were frozen and submitted to the OSU Central Analytical Lab for analysis of nitrate and ammonium content. The N source for all trials was urea. Post-harvest soil samples were obtained on 14 September.

Beans

The plot area received a broadcast, incorporated application of 0.75 pounds trifluralin, 2.0 pounds EPTC, and 1.2 pounds chlorpyrifos/acre. 'Oregon 91G' green beans were seeded at 65 pounds/acre on 9 May with four rows per plot on 30-inch centers. Triple superphosphate (130 pounds/acre) was banded at planting two inches beneath and two inches to the side of the seed row. The first 40 pounds N/acre was broadcast at planting; the remaining N was broadcast on 6 June. Plots were sprinkler-irrigated and cultivated as necessary and harvested on 25 July. Plants were mowed to simulate machine-harvest and minimize further N uptake.

Beets and Carrots

The plot area received a broadcast, incorporated application of EPTC at 2.0 pounds/acre (beets) and 150 pounds triple superphosphate/acre. Carrot plots were treated with linuron at 1.25 pound/acre one day after seeding. 'Detroit Dark Red' table beet and 'Orlando Gold' carrot were seeded on 5 May with three rows on a five-foot bed. The first 40 pounds N/acre was broadcast on the planting date; the remaining N was broadcast on 10 June. Plots were sprinkler-irrigated and cultivated as necessary and harvested on 25 July (beets) and 16 August (carrots). The remaining beet plants were pulled to minimize further N uptake.

Results and Discussion

Green beans responded with a yield increase to the first increment of applied N, but yield did not increase further with rates of N application greater than 40 pounds/acre (Table 29). This is somewhat unusual, as most experience with this crop indicates increasing yield up to 100 pounds applied N/acre. Carrot yield responded as expected, with the greatest yield at 120 pounds N/acre (Table 29). This is consistent with results previously obtained at NWREC. Beet yield increased markedly with the first increment of applied N and continued to increase in quadratic fashion with the maximum at 240 pounds N/acre (Table 30). This is also consistent with our previous research and with grower experience.

Soil nitrate concentration after bean harvest increased approximately linearly with increasing rate of applied N at the 1 and 2 foot depths, but the effect was significant only in the surface foot of soil. Soil ammonium content also increased with increasing applied N for the surface foot of soil (Table 31).

Soil ammonium concentration did not differ with rate of applied N for beet and carrot, but in each case soil nitrate concentration increased at higher rates of applied N for the first two feet of the soil profile (Tables 32 and 33). For carrot, there was no increase in soil nitrate content in the surface foot of soil until the applied N reached the optimal level for yield (Table 33). In the beet plots, the greatest soil nitrate content in the surface two feet of soil was with 180, rather than 240, pounds applied N/acre (Table 32). The same trend, although not significant, was apparent in the residual soil ammonium content in the beet plots. Further research will be needed to determine whether this observation was simply due to chance.

Table 29. Effect of rate of urea-nitrogen on the 
yield of green beans and carrots, NWREC, 1994     
N rate             Bean           Carrot         
(lb/A)             (T/A)           (T/A)          
  0		        4.5            15.8	
 40		        7.5            21.0
 80		        6.8            23.1
120		        6.7	       26.0
160		        7.3	       24.0
     Significance       L*Q*           L*Q*     
L=linear, Q=quadratic, *significant, p=0.05.


Table 30. Effect of rate of urea-nitrogen  
on the yield of table beets, NWREC, 1994 
N rate                  Yield
(lb/A)                  (T/A)            
  0                       3.7
 60                      13.6
120                      16.4
180                      17.6
240                      18.0
      Significance      L**Q*            


Table 31. Effect of rate of broadcast nitrogen on soil nitrate and ammonium
concentrations (ppm) at four depths following bean harvest, NWREC, 1994     
                                            N rate, lb/A                      
Sample depth (inches)                  0    40    80   120    160   LSD(.05)
                         Pre-plant   --------Post-harvest--------         
Nitrate
 0-12                       0.7      1.6   7.7  10.2   9.9   16.4   14.1 
12-24                       0.7      1.0   2.7   3.9   3.7    5.3    NSD  
24-36                       1.6      1.3   2.3   5.5   3.4    3.8    NSD  
36-48                       2.3      1.3   2.3   5.0   3.9    3.1    NSD
Ammonium
 0-12                       1.9      3.4   4.8   3.9   6.0    7.0    2.0 
12-24                       1.9      2.7   2.3   3.1   2.9    3.2    NSD
24-36                       2.0      2.9   1.7   4.9   2.6    2.7    NSD
36-48                       1.8      3.0   1.7   4.0   3.6    2.7    NSD   


Table 32. Effect of rate of broadcast nitrogen on soil nitrate and ammonium
concentrations (ppm) at four depths following beet harvest, NWREC, 1994    
Sample depth (inches)                       N rate, lb/A       
                                      0    60   120   180   240  LSD(0.05)  
                        Pre-plant   --------Post-harvest-------
Nitrate
 0-12                      1.5      0.8   1.4   3.0   6.6   3.9    1.7   
12-24                      1.2      0.3   0.6   1.0   1.9   1.6    0.6   
24-36                      1.1      0.9   1.2   2.0   1.6   1.9    NSD   
36-48                      1.0      1.9   2.9   3.3   2.2   4.4    1.9
Ammonium
 0-12                      0.8      2.6   3.0   5.3   6.1   4.1    NSD
12-24                      2.7      3.1   2.5   3.2   5.5   3.2    NSD
24-36                      3.7      3.2   2.4   3.6   4.2   2.0    NSD
36-48                      3.3      3.3   2.4   3.8   2.7   2.0    NSD     


Table 33. Effect of rate of broadcast nitrogen on soil nitrate and ammonium
concentrations (ppm) at four depths following carrot harvest, NWREC, 1994  
                                             N rate, lb/A                   
Sample depth (inches)                  0    40    80   120    160  LSD(.05)
                        Pre-plant    --------Post-harvest--------        
Nitrate
 0-12                       1.5      0.8   0.4   0.9   5.5    8.2    6.6    
12-24                       1.2      0.4   0.5   1.6   1.9    1.5    1.0   
24-36                       1.1      1.6   1.7   3.8   3.8    2.8    NSD   
36-48                       1.0      2.1   2.9   3.7   3.2    3.0    NSD
Ammonium
 0-12                       0.8      2.3   3.2   2.8   2.7    2.4    NSD 
12-24                       2.7      2.6   3.5   2.3   2.9    2.4    NSD
24-36                       3.7      2.5   3.5   2.0   3.9    2.8    NSD
36-48                       3.4      2.7   4.9   2.4   2.6    2.6    NSD   

Post-harvest Mineral Nitrogen Status in Grower Fields

Cooperators: John Hart, Department of Crop and Soil Science
N.S. Mansour and John Luna, Department of Horticulture

Introduction

This grower trial was undertaken to determine whether residual nitrate and ammonium levels in grower fields were similar to those found in our experiments at NWREC. The data should be useful in indicating which of the major processed vegetable crops leave significant quantities of residual mineral N in the soil at harvest and the extent to which grower cultural practices, particularly fertilizer application, influence the amount of residual N that is available for leaching by heavy winter rainfall.

Methods

Soil samples were taken in one-foot increments to a depth of 5 feet both before and after crops of green beans, beets, broccoli, carrots, cauliflower, and sweet corn, for determination of mineral N (ammonium-N and nitrate-N) content. Thirty fields were sampled in 1993 and 34 in 1994, representing 15 growers and 7 soil types. The growers were interviewed to determine field history and cropping and fertilization intentions and they kept records of fertilizer applications and irrigations.

Results and Discussion

Mean pre-plant nitrate and ammonium concentrations for 1993 are seen in Tables 34 and 35, respectively. Corresponding data for 1994 are in Tables 36 and 37. In order to preserve anonymity, only means are presented. However, there were few surprises in the pre-plant data. The relatively heavy rainfall during the late winter and spring resulted in low levels of nitrate in most fields in both years. Most individual cases of higher levels of nitrate or ammonium could be explained by a past history of manure application or by the presence of a legume cover crop.

Mean nitrate and ammonium levels at harvest varied by crop (Tables 34-37) and grower cultural methods (data not shown). For example, in top foot of the soil, nitrate concentrations were greater for sweet corn than for cauliflower or broccoli, although the mean N application to these crops was 203, 226, and 262 pounds/acre, respectively, in 1993 and 193, 223, and 253 pounds/acre, respectively, in 1994 (Tables 34 and 36). Nitrate levels were generally elevated for sweet corn, not only in the surface foot of soil, but also at greater depths. This contrasts with our experience at NWREC and may indicate movement of nitrate as a result of the unusually wet late spring and early summer, or may indicate that improvements could be made in grower irrigation practices. For carrots, the elevated nitrate levels at the 12 to 36-inch depths may indicate soil mixing during digging. Post-harvest ammonium concentrations varied much less between pre-season and post-harvest sampling than did nitrate (Tables 35 and 37). Presumably, this is due to conversion of urea and ammonium-N to nitrate during the growing season.


Table 34. Mean post-harvest nitrate concentration (ppm) in grower
fields as a function of crop and depth in the soil profile, 1993 
Crop                  Depth of sample (inches)    
                  0-12     12-24    24-36    36-60               
Pre-plant          4.2      2.7      2.5      2.5        
Beans             13.9      8.0      6.4      5.7
Beets             15.3      4.4      2.8      3.2
Broccoli          10.4      3.9      2.5      2.7
Carrots            9.2     11.8      7.1      4.3
Cauliflower       15.3      6.8      5.0      5.5
Sweet corn        21.9     10.0      8.8      7.2                


Table 35.  Mean post-harvest ammonium concentration (ppm) in grower
fields as a function of crop and depth in the soil profile, 1993   
Crop                  Depth of sample (inches)       
                  0-12     12-24    24-36    36-60                 
Pre-plant          3.6      2.9      2.6      2.6
Beans              4.8      3.2      3.1      2.7
Beets              4.6      3.1      3.1      2.7
Broccoli           4.8      5.0      3.1      3.4
Carrots            1.0      0.3      0.3      0.1
Cauliflower        6.7      4.1      3.5      3.8
Sweet corn         4.3      3.2      3.3      3.8                  


Table 36. Mean post-harvest nitrate concentration (ppm) in grower
fields as a function of crop and depth in the soil profile, 1994 
Crop                  Depth of sample (inches)       
                  0-12     12-24    24-36    36-60               
Pre-plant          5.0      4.6      5.5      5.2        
Beans             20.2      5.8      7.1      6.6
Beets             13.3      6.1      4.1      3.2
Broccoli           7.2      3.0      2.3      1.9
Carrots           12.8      7.5      7.4      6.3
Cauliflower        9.9      6.9      6.9      6.8
Sweet corn        22.2      9.1      9.6      8.0                


Table 38.  Mean post-harvest ammonium concentration (ppm) in grower
fields as a function of crop and depth in the soil profile, 1994    
Crop                  Depth of sample (inches)       
                  0-12     12-24    24-36    36-60                 
Pre-plant          4.2      3.9      3.0      4.1
Beans              7.4      3.5      4.0      3.6
Beets              4.5      3.6      3.0      2.2
Broccoli           6.0      4.7      3.5      3.0
Carrots            3.1      2.5      1.9      1.5
Cauliflower        7.2      3.6      3.2      4.0
Sweet corn         4.7      3.1      2.6      2.7                  

Effect of Nitrogen Rate and Cover Crops on the Yield of Sequential Crops of Broccoli and Sweet Corn

Cooperator: Richard Dick, Dept. of Crop and Soil Science

Introduction

Nitrate pollution of groundwater from the application of high rates of nitrogen fertilizers to vegetable crops in an increasing concern in the Willamette Valley. Excess nitrogen not taken up by the crop remains in the soil and can be leached to groundwater during the wet winter months. These concerns led us to initiate a study of the cycling and availability of nitrogen in vegetable cropping systems. These are the fourth and fifth years of a study in which winter cover or "catch" crops have been seeded following vegetable crops and in which the N uptake of the cover crop and its contribution to a succeeding vegetable crop has been measured. In 1991, broccoli was grown on the long-term rotation plots at NWREC and fertilized at three rates of nitrogen. Following harvest the plots were seeded to cereal rye or a mixture of cereal rye and Austrian winter pea. In 1992, sweet corn was grown on these plots at three rates of nitrogen to determine the cover crop contribution to sweet corn yield and nitrogen uptake. Following harvest, the plots were again disked, harrowed, and seeded (drilled) to the above cover crops. In addition, other plots were overseeded to cereal rye or red clover about one month after sweet corn emergence. These cover crops were permitted to grow through the winter. In 1993, broccoli was again grown on the plots and fertilized with two or three rates of applied nitrogen. In 1994, we again grew sweet corn following the 1993 broccoli crop and cover crops as well as following spring and fall-plowed clover.

Methods

The overseeded cover crops of 'Kenland' red clover and 'Wheeler' cereal rye were broadcast on 1 July, 1992, and 15 July, 1993, into four plots each of the standing sweet corn (1992) or broccoli (1993) crops. The direct-seeded cover crops were seeded on 14 October, 1993, and 7 October, 1994, after disking and harrowing to form a seedbed. These plots had been cropped to sweet corn in 1990, broccoli in 1991, and sweet corn in 1992, with N rates of 0, 50, and 200 pounds/acre for sweet corn and 0, 125, and 250 pounds/acre for broccoli. Four plots (30 x 60 feet) were planted to 'Wheeler' cereal rye at 65 pounds/acre. The other four plots were planted to a mixture of 'Wheeler' rye at 35 pounds/acre and Austrian winter pea at 100 pounds/acre. No fertilizers or pesticides were applied to the cover crops. Nitrogen rate subplots of 600 square feet each were determined by the nitrogen applied to the vegetable crop. The identity of the three N rate subplot treatments was maintained from year to year.

On 14 April of both years, samples were taken from all subplots for determination of shoot dry weight and nitrogen uptake. The shoots were clipped about one inch above ground. All cover crops were mowed down on 5 May, 1993 and 15 April, 1994. The plots were plowed, disked, and harrowed in early May. In addition, plots which had been in 'Kenland' red clover as a seed crop were either fall- or spring-plowed and prepared for planting broccoli or sweet corn.

On 19 May, 1993, 1.3 pounds chlorpyrifos and 10.0 pounds Solubor/acre were applied to the plots which had been overseeded. The plots which had been in drilled rye or rye plus plea or were fallowed or had been in clover as a seed crop were treated with the above plus trifluralin at 0.75 pounds/acre. The pesticides were rototilled into the surface three inches of soil. 'Gem' broccoli was seeded in 20-inch paired rows on 9 June. The distance between pairs of rows was 40 inches.

On 16 June, 1993, nitrogen was applied as urea in a surface band between the paired rows at rates of one-half the total N rates of 0, 125, and 250 pounds/acre. Subplots of the clover seed crop plots received only the 0 and 250 pound rates of total N. Drip irrigation tubing was then installed between each pair of rows. On 28 June, the broccoli was thinned to a stand of 10 inches between plants in the row. The remainder of the urea was sidedressed on 21 July, at which time the appropriate treatments were again overseeded with rye or clover, using a whirly-bird fertilizer spreader. The seed was scratched in with a garden rake.

Plots were tractor-cultivated on 13 July, 1993, and hoed as necessary later in July. All plots were harvested on 19 August and again on 30 August from 15 feet of two inner rows of each subplot. Following harvest, the appropriate plots were again prepared for planting of cover crops.

On 23 May, 1994, 'Jubilee' sweet corn was seeded in 20-inch paired rows with 40 inches between pairs of rows. Phosphorus was banded at 60 pounds P2O5/acre two inches to the side and two inches beneath the seed row. Plots which had been in overseeded rye or clover were treated with EPTC at 3.0 pounds/acre, which was incorporated before planting. All other plots received a broadcast application of 2.0 pounds atrazine and 3.0 pounds alachlor/acre immediately after planting.

On 6 June, 1994, nitrogen was applied as urea in a surface band between the paired rows at rates of one-half the total N rates of 0, 50, and 200 pounds/acre. Subplots of the clover seed crop plots received only the 0 and 200 pound rates of total N. All N rate subplots were in the same location as the corresponding N treatments on the previous vegetable crops. Drip irrigation tubing was then installed between each pair of rows. The remainder of the urea was sidedressed on 1 July, at which time the EPTC-treated plots were again overseeded with rye or clover, using a whirly-bird fertilizer spreader. EPTC-treated plots were hand-hoed as necessary before overseeding. Harvest on 31 August was from 15 feet each of the two innermost rows of each subplot. Following harvest, the appropriate plots were again prepared for planting of cover crops.

Results and Discussion

Both increasing the fertilizer rate on the preceding vegetable crop and the presence of peas in the cover crop increased total cover crop yield and nitrogen uptake (Tables 39 and 40). In 1994, the dry matter yield of overseeded rye or overseeded clover was approximately one-half that of drilled rye. This is in contrast to 1993, when the overseeded crops yielded about the same as the drilled cover crops. In 1993, the greatest cover crop biomass occurred on clover plots which had been harvested for a seed crop and allowed to regrow since the summer of 1992 (clover green manure). In 1994, however, the yield of a clover green manure was comparatively poor, due to a poor stand of the seed crop. Nitrogen uptake in 1993 was greatest for the clover green manure and least for rye. Nitrogen uptake by rye increased with increasing rate of nitrogen applied to the previous vegetable crop in 1993, particularly for the high rate of nitrogen (Table 39). Legume nitrogen uptake did not vary with rate of applied N in 1993. Peas contributed relatively little N uptake to the total for plots seeded to rye plus plea; however, rye N uptake also increased in the presence of peas. Nitrogen uptake of the overseeded clover did not vary with rate of applied N in 1994 (Fig. 1). Yield of overseeded clover also did not vary with preceding N rate (data not shown).

Fig. 1
A rough estimate of the amount of residual fertilizer N left over from the broccoli crop that was recovered by the rye cover crop can be obtained by examining the rye-only uptake at the three fertilizer rates as shown in Figures 2 and 3. For 1993, subtracting the amount of N taken up by the drilled rye grown on non-fertilized subplots from the N taken up at the other two N rates suggests that only 5 pounds N/acre (1 pound for overseeded rye) was taken up from the intermediate rate of N and 29 pounds/acre (25 for overseeded rye) from the high rate of N. Likewise, in 1994, this process suggests that about 20 pounds N/acre (6 pounds for overseeded rye) was taken up from the intermediate rate of N and 28 pounds/acre (29 for overseeded rye) from the high rate of N. This nitrogen would have been available for leaching. Of course, an undetermined amount of nitrogen may have leached before the cover crops were well established. The overseeded rye did not achieve much germination or growth until after the vegetable crop was mowed down and the onset of fall rains. Stands were poor. Thus, it is not surprising that the yield and N uptake for the overseeded rye are less than for the drilled rye in 1994. Cover crop N recovery in 1993 was similar, except that stands of overseeded covers were better and yield and N uptake was about equal at all N rates.

Fig. 2.
Fig. 3
Broccoli yield in 1993 varied significantly with cover crop and nitrogen rate (Table 41). Yield of broccoli on plots which had been in rye was slightly depressed compared to plots which had been winter fallowed. This is consistent with results obtained in 1991. Among possible explanations are the possibility of allelopathy from the rye residue, immobilization of mineral N by the decomposing rye straw, or an adverse effect of cereal rye on soil tilth. When a legume was present in the cover crop, broccoli yields tended to be greater than for the winter fallow, but the differences were not significant. Greatest yield and mean head weight were from plots which had been in overseeded clover.

In contrast to sweet corn and broccoli yields in 1990 through 1992, the yield of broccoli did not tend to increase from the intermediate to the highest rate of nitrogen. This was the case for nearly every combination of cover crop treatment and N rate (Table 42). A buildup of soil organic N due to cover cropping, thus eliminating the need for high rates of nitrogen might be an explanation for the lack of response to a rate of N previously found to be optimal for broccoli at this site. However, since the plots which had been winter fallowed for four years responded similarly, this does not appear to be a valid explanation. The greatest yield recorded in this trial was for the combination of an overseeded clover cover crop and the intermediate rate of N.

Sweet corn yield in 1994 also varied significantly with cover crop and nitrogen rate (Table 43). There were no significant interactions of cover crop and N rate affecting any component of yield or quality, so only main effects of cover crop and N rate are shown. As in 1993, yield from plots which had been in drilled rye was depressed compared to any other treatment except the overseeded rye. The combination of cereal rye and winter pea produced yield equal to that following winter fallow and the greatest mean ear weight, while overseeded clover or a spring-plowed clover seed crop significantly increased yield compared to winter fallow, presumably due to the nitrogen contribution from the clover.

Sweet corn yield, number of ears harvested, ear length, tipfill, and mean ear weight all increased with each increase in rate of applied N, regardless of cover crop treatment (data not shown). This is consistent with results obtained in 1990 through 1992 for sweet corn and broccoli. However, in 1993, broccoli yield did not increase from the intermediate to the highest rate of N. In 1994, the greatest yield (8.8 tons/acre), number of ears harvested (28,170/acre), tipfill (3.5), and mean ear weight (284 g) were with the combination of overseeded clover and the greatest rate of applied N.

The contribution of a cover crop legume to broccoli or sweet corn yield can best be appreciated by comparing yield at zero applied N (Fig. 4 and 5). While the broccoli yield from the rye cover crop plots was the same or slightly depressed compared to fallow, the rye plus pea, overseeded clover, and spring-plowed clover green manure crop all significantly increased yield.

Fig. 4Fig. 5
The difference in yield between corn on fallowed plots with no applied N and that from fallowed plots with 50 pounds fertilizer N/acre was 4.0 tons/acre. The plots which had been in overseeded clover, but which received no fertilizer, produced 3.5 tons/acre greater yield than did the unfertilized fallowed plots. Assuming linear response to available N over this range, the yield increase of 3.5 tons/acre indicates 44 pounds N/acre were provided by the overseeded clover. Similarly, the spring-plowed clover seed crop provided an estimated 34 pounds N/acre. The rye or rye-pea cover crops did not appear to provide N to the sweet corn crop.


Table 39. Main effects of cover crop species and nitrogen rate on previous sweet 
corn crop on the yield and nitrogen accumulation by cover crops, NWREC, 1993    
Treatment         Dry yield   Rye N uptake   Legume N uptake   Total N uptake   
                  -------------------------lb/acre---------------------------
Cover crop
Drilled rye          2421         34                0                34
Drilled rye+pea      2904         45               12                57
Overseeded rye       2593         38                0                38
Overseeded clover    2510          0               62                62
Clover green manure  3548          0              105               105
      LSD (0.05)      601         10               15                12
N rate (lb/A)z
  0                  2167         27               38                39
 50                  2325         32               34                41
200                  3329         58               37                62
      Significance    **          **               NS                **         
zDoes not include means for the clover green manure crop, to which N was 
 not applied.


Table 40. Main effects of cover crop species and nitrogen
rate on previous broccoli crop on the yield and nitrogen
accumulation by cover crops, NWREC, 1994                 
Treatment         Dry yield    N uptake                     
                  -------lb/acre-------
Cover crop
Drilled rye          1915         32   
Drilled rye+pea      2135         50   
Overseeded rye       1067         20   
Overseeded clover     996         34   
Clover green manure   740         27   
      LSD (0.05)      547         10   
N rate (lb/A)z
  0                  1032         25
125                  1621         33
250                  1932         44
      Significance    **          **                    
zDoes not include means for the clover green manure 
 crop, to which N was not applied.


Table 41. Main effects of preceding cover crop, and rate 
of applied N on yield and quality of broccoli, NWREC, 1993
Treatment                  Yield        Mean head 
                           (T/A)         wt. (g)            
Cover crop
Fallow                      3.3            202        
Rye                         2.8            180
Rye + pea                   3.6            208
Overseeded rye              3.1            203
Overseeded clover           3.7            241
Fall-plowed clover          3.1            197
Spring-plowed clover        3.6            221
	      LSD (0.05)    0.6             35
N rate (lb/A)
  0                         2.4            149
125                         3.9            233
250                         3.7            238
              LSD (0.05)    0.4             27            


Table 42. Interaction of preceding cover crop and rate of 
applied N on yield and quality of broccoli, NWREC, 1993   
Cover crop              N rate       Yield       Mean head 
                        (lb/A)       (T/A)        wt. (g) 
Fallow                     0          1.9           124
                         125          4.1           246
                         250          3.9           236
Rye                        0          1.6           111
                         125          3.4           208
                         250          3.3           221
Rye + pea                  0          3.5           184
                         125          3.7           210
                         250          3.7           229
Overseeded rye             0          2.0           138
                         125          3.5           221
                         250          3.7           249
Overseeded clover          0          2.9           190
                         125          4.5           277
                         250          3.7           256
Fall-plowed clover         0          2.4           157
                         250          3.8           237
Spring-plowed clover       0          3.2           188
                         250          3.9           254
                     LSD (0.05)       1.1            61    


Table 43. Main effects of preceding cover crop, and rate of applied nitrogen 
on yield and quality of sweet corn, NWREC, 1994                              
Treatment                  Yield   No. ears    Mean ear   Ear length  Tipfill
                           (T/A)  harvested/A   wt. (g)    (inches)           
Cover crop
Fallow                      5.1      22120        205        8.0        2.7
Fallow, clover in 1992      5.1      23090        201        7.8        2.6
Rye                         3.7      18050        177        7.6        2.2
Rye + pea                   5.2      23620        256        8.4        2.9
Overseeded rye              4.4      20720        172        7.5        2.1
Overseeded clover           7.1      24880        192        8.0        2.4
Fall-plowed clover          5.8      21930        228        8.4        2.9
Spring-plowed clover        6.3      23670        230        8.4        2.8
	      LSD (0.05)    1.1       2460         37        0.5        0.5
N rate (lb/A)
  0                         3.3      18000        156        7.4        1.9
 50                         5.2      23380        211        8.0        2.5
200                         7.1      25830        253        8.6        3.3
              LSD (0.05)    0.7       2590         18        0.3        0.3  

Effect of Frequency and Timing of Irrigation on Yield and Head Rot of Broccoli

Cooperator: Mary L. Powelson, Dept. of Botany and Plant Pathology

Introduction

Bacterial soft rot, caused by Erwinia carotovora and possibly by Pseudomonas species, often affects broccoli production in the Willamette Valley. The rot can affect floret or stem tissue and occurs mainly during periods of moderate temperatures and high humidity or rainfall. Chemical controls are ineffective and resistance has not been identified in varieties well-adapted to the local climate and acceptable to processors. While reductions in planting density may somewhat reduce disease incidence, the decreased disease incidence is not sufficient to offset the loss in number of heads harvested.

Previous work at NWREC (R. Ludy, M.S. thesis, Oregon State University, 1990) indicated that the amount of water applied to a broccoli crop did not affect floret soft rot (head rot) incidence or severity, at least within a range of applied water that allowed for economic yields. However, in non-replicated observations of the effect of irrigation frequency (once versus three times per week), head rot incidence more than doubled under the greater frequency. We propose that the incidence of rot is affected by the frequency and duration of water droplets on the floret and stem tissue. The purpose of these trials was to determine the effects of two or three frequencies of irrigation and the time of day of the irrigations, at equal amounts of applied water, on yield and head rot incidence in broccoli.

Methods

'Gem' broccoli was seeded to a Willamette silt loam, Ph 6.0, on 7 July, 1993 and 6 July, 1994. In 1993, the experimental design was a split-plot with a 2x2 factorial combination of irrigation frequency (every second and every eighth day) and timing (morning and evening) as main plots and harvest as subplot treatments. In 1994, the design was a factorial combination of three frequencies (every second, fourth, and eighth day) and the same timings. The main plots in 1993 consisted of 12 30-foot rows (18 in 1994), with three rows on a 60-inch bed. The subplots consisted of individual three-row beds. Main plot treatments were replicated four times in a completely random design. Plot preparation included a broadcast and incorporated application of 1000 pounds 10-20-20 fertilizer/acre, 0.75 pounds trifluralin, 1.3 pounds chlorpyrifos, and 10.0 pounds Solubor/acre. The area was then rototilled and cultimulched to form a seedbed. Plots were seeded with a gang of three tractor-mounted Planet Jr. planters. Broccoli was thinned to about 10 inches between plants during the last week of July. Diazinon was applied at 2.0 pounds/acre for maggot control in early August. In 1993, eighty pounds N/acre, as ammonium nitrate, was sidedressed on 17 August. In 1994, 100 pounds N/acre, as urea, was sidedressed on 2 August and an additional 50 pounds N/acre was sidedressed on 1 September.

Plots were irrigated uniformly as needed, about 4 cm/week, until 17 September, 1993 (8 September, 1994) when the irrigation treatments were initiated. Most plants had 1-inch diameter heads at this date. The irrigation system was constructed such that each of the treatment combinations was controlled by a separate valve with flexible hose used to connect the four plots making up the replicates of each treatment. Water was applied from a sprinkler located at each end of the main plots. Main plots were isolated from each other by 30 feet in all directions. Sprinklers were set to water a 180-degree pattern.

A spontaneous mutant of Erwinia carotovora subsp. carotovora (EccW3C105) was obtained from J.E. Loper, USDA, Corvallis, OR and selected for field trials. It was resistant to 100 g/mL nalidixic acid and phenotypically similar to the parental strain. Lawns of the Erwinia isolate were grown on Lauria Bertani (LBnal) media (10 g Bacto tryptone, 5 g Bacto yeast extract, 10 g NaCl, 20 g Bacto agar in 1.0 liter of distilled H2O with the addition of 100 g/mL nalidixic acid) for 3 days. The bacteria were washed from plates and the concentration of inoculum was adjusted to 107 cells/mL. On 16 and 23 September at 8 am and 7 pm, respectively, plots were sprayed with the bacterial suspension in 25 gal water/acre using a CO2-pressurized boom sprayer with three flat fan nozzles.

To determine the survivability of Erwinia over time, broccoli florets were sampled every other day at 9:00 am from 17 September to 8 October, 1993. Additionally, florets were sampled two hours after the first bacterial application and the morning following the second application. A five-foot section across each main plot (approximately 48 plants) was used for sampling. Five samples (approximately 0.2 g of floret tissue) per head were collected from three heads in each plot. Individual samples were placed in separate test tubes containing 2.24 mL of sterile potassium phosphate buffer (ph 6.5, 0.05 M). Test tubes were agitated and then racks of test tubes were sonicated for 5 min. After agitating again, a 0.01 ml aliquot of the wash and a 1:22.4 dilution of the wash were each plated on separate halves of plates filled with LBnal medium. Minimum detection limits for bacteria in the wash and the dilution were 2.24 x 102 and 5.0 x 103, respectively. Bacterial populations were counted three days after plating.

The duration of the irrigation treatments, one-half, one, or two hours, was intended to deliver 1 cm of water for the 2-day frequency, 2 cm for the 4-day, and 4 cm for the 8-day frequency, respectively. Rain gauges were located in the center of each main plot; actual water accumulation was measured immediately after each irrigation. Preliminary measurements at less than 1 knot winds indicated excellent uniformity of water application (less than 0.1 cm variation) both among plots of the same treatment and within main plots. The half-hour irrigations commenced at 8:30 a.m. (morning) or 6:30 p.m. (evening). The longer irrigations commenced at correspondingly earlier times, with all irrigations terminating at 9 a.m. or 7 p.m.

Soil moisture content of the surface 15 cm of the soil profile, as a percentage, was measured by time-domain reflectometry at about 2 p.m. each day from 17 September until 1 October, 1993 and on 19 and 21 September, 1994. Leaf wetness duration was measured with a Campbell Scientific 21X micrologger (Campbell Scientific, Logan, UT) in four plots representing each treatment. Four replicate sensors attached to ringstands were placed in each plot in the center of the canopy. Sensors were sampled at 30-minute intervals and wetness duration was computed as the percentage of the interval that the sensor was wet.

Visual disease ratings were made at four-day intervals in a three-row subplot in each plot. Heads were harvested from the appropriate subplots on 28 September, and 4 and 8 October, 1993 (19 and 29 September, 1994), counted, weighed, and checked for disease incidence.

Results and Discussion

In 1993, mean weight of broccoli heads at the first harvest was greater for the 2-day irrigation interval than for the 8-day interval and tended to be greater for the morning as opposed to evening irrigation (Table 44). At the second harvest mean head weight was nearly identical between frequencies, but was greater for the evening irrigation (Table 45). Most of these differences appeared to be due to delayed maturity and small heads for one replicate of the 8-day x morning treatment combination. The same trend was not observed at the third harvest (Table 46) and mean head weight did not vary with treatment for the sum of all harvests (Table 47). Since there were no significant interactions of irrigation frequency and timing affecting yield or rot incidence, only main effects have been presented in the tables.

The incidence of head rot in 1993 was very low, less than 10 percent. Irrigation treatment had no effect on rot incidence at the first harvest, but there was a greater incidence of rot with the evening irrigations at the second harvest. At the third harvest, the 2-day interval produced more rotten heads and, although not significant, the evening irrigation still tended to produce more rot than did the morning irrigation. For the sum of all harvests, only the effect of irrigation interval was statistically significant. In the observational sub/plots that were not harvested, only three heads were observed with head rot on 4 October. Four days later, on 8 October, the number and percentage of heads with rot was significantly greater in the 2-day irrigation interval treatment compared with the 8-day interval. The incidence of head rot did not differ significantly between morning and evening irrigations, although incidence was greater in the evening-irrigated plots.

Populations of the Erwinia mutant were recovered from all of the heads sampled two hours after the first inoculation and varied from 102 to 105 colony forming units (cfu)/g fresh weight of tissue. Two and four days later, colonies were recovered from only 2 and 3 plots, respectively. After the second inoculation, bacteria were recovered from most of the heads with populations ranging from 102 to 105. Recovered Erwinia populations immediately began to decline. Two, four and six days later, bacteria were recovered from 29, 12 and 10% of the heads, respectively. No bacteria were recovered after 30 September. Several possible reasons for the sharp decline are proposed. Environmental conditions were unfavorable to establishment and survival of the bacteria at the time of and immediately following application. Weather conditions prevalent during the sampling period were characterized by dry and warm days. Bacteria applied to aerial portions of plants are susceptible to desiccation. The ability of the Erwinia mutant to survive in situ has not been fully investigated. Due to the poor survival of the applied mutant, an analysis of treatment effect on survival of bacteria could not be made.

Several bacteria were isolated from diseased heads, including several Psuedomonads and undetermined isolates, but no Erwinia. These bacteria will be tested for their ability to cause head rot on broccoli.

Total precipitation between seeding and initiation of the irrigation treatments was 8 cm in 1993. During the irrigation treatments only 0.15 cm precipitation was recorded and probably had little influence on the treatments. The weather was unusually warm and dry during most of the treatment period. However, temperatures returned to normal seasonal averages during the last week of the experiment, with mostly cloudy skies.

Averaged over the length of the treatments, soil moisture did not vary with treatment if only the measurements taken on the no-irrigation days are used (Table 48). Since the soil moisture readings were taken midway between the morning and evening irrigations, the readings were greater for morning than for the evening-irrigated plots, if measured on the day of irrigation (data not shown). Soil moisture for the 2-day irrigation interval tended to be fairly constant over time, although there was an increase late in the experiment, perhaps related to greater cloud cover, reduced temperatures, and reduced evapotranspiration (Fig. 6). For the eight-day interval, soil moisture was much less constant. Soil moisture readings were terminated on 1 October because of instrument failure.

Fig. 6
The lack of a consistent treatment effect on yield, and the lack of effect of treatment on soil moisture and average amount of water applied to each plot, indicate that the effects of treatment on head rot incidence are due to frequency or timing of irrigations rather than to the amount of water applied. Although timing and frequency did not affect the total amount of water applied or average soil moisture, it did affect leaf wetness duration. Mean hours of leaf wetness per day over the course of the study was greater in the two-day irrigation interval compared to the eight-day irrigation interval. The effect of timing was not significant, although plots irrigated in the evening tended to have greater average daily hours of leaf wetness (Table 49). Across all treatments, average daily hours of leaf wetness increased as the season progressed. The last three days of September, leaf wetness averaged 12.9 hours per day and by the final three days of the study leaf wetness averaged 19.9 hours per day.

In 1994, the amount of water (0.68 cm/day) applied to the plots during the treatment period slightly exceeded the goal of 0.5 cm/day. However, this included two occurrences of precipitation, averaging 0.07 cm/day. The amount of water applied did not vary significantly with treatment. Mean soil moisture also did not vary with treatment (Table 50).

Mean weight of broccoli heads and yield per unit area in 1994 did not vary with either frequency or timing of irrigation at either harvest (Tables 51 and 52). The first harvest occurred during a period of unusually hot, dry weather and no rot was observed. Since there were no significant interactions of irrigation frequency and timing affecting yield or rot incidence, only main effects have been presented in the tables.

The incidence of head rot was fairly high at the second harvest, averaging 22 percent for all treatments (Table 52). As in 1993, incidence of rot decreased with increasing interval between irrigations but timing of the irrigation had no effect.

Taken together, the 1993 and 1994 results support the hypothesis that development of disease is related to the availability and duration of free moisture. The data also provide evidence that irrigation practices may be useful in disease control. Avoiding frequent irrigation and timing irrigations to allow rapid drying of plants may reduce rot incidence.

Table 44. Main effects of frequency and timing of irrigation on yield and 
head rot incidence in broccoli at first harvest, NWREC, 1993             
Treatment     No. heads harvested    Yield    Mean head      No. rotten
                  per plot          kg/plot    wt. (g)     heads per plot
Frequency
2-day interval      50.0             11.5        231           0.2          
8-day interval      45.7              8.2        182           0.0
    Significance     NS               NS          *             NS
Timing
morning             46.5             10.2        220           0.0
evening             49.2              9.4        194           0.2
    Significance     NS               NS          NS            NS        


Table 45. Main effects of frequency and timing of irrigation on yield and 
head rot incidence in broccoli at second harvest, NWREC, 1993            
Treatment     No. heads harvested    Yield    Mean head      No. rotten
                  per plot          kg/plot    wt. (g)     heads per plot
Frequency     
2-day interval      61.1             18.1        296           1.9
8-day interval      60.8             18.2        299           0.5
    Significance     NS               NS          NS            NS
Timing
morning             62.9             17.1        272           0.3
evening             59.0             19.2        325           2.2
    Significance     NS               NS          *             *        


Table 46. Main effects of frequency and timing of irrigation on yield and 
head rot incidence in broccoli at third harvest, NWREC, 1993              
Treatment     No. heads harvested     Yield    Mean head      No. rotten
                  per plot           kg/plot    wt. (g)     heads per plot
Frequency
2-day interval      56.0              23.2        415          12.3 
8-day interval      54.5              22.1        403           5.5
    Significance     NS                NS          NS            *
Timing
morning             59.0              23.9        409           8.1
evening             51.5              21.4        410           9.7
    Significance     NS                NS          NS            NS       


Table 47. Main effects of frequency and timing of irrigation on yield and 
head rot incidence in broccoli, sum of all harvests, NWREC, 1993          
Treatment     No. heads harvested     Yield    Mean head      No. rotten
                  per plot           kg/plot    wt. (g)     heads per plot
Frequency
2-day interval     167.1              52.7        317          14.4 
8-day interval     161.0              48.5        300           6.2
    Significance     NS                NS          NS           **
Timing
morning            168.4              51.2        303           8.6
evening            159.7              50.1        313          12.0
    Significance     NS                NS          NS            NS       


Table 48. Main effects of frequency and timing of irrigation on the 
mean amount of water applied per day and on the mean percent soil 
moisture during the course of the experiment, NWREC, 1993          
Treatment            Water applied, cm/day       Soil moisture (%) 
Frequency
2-day interval              0.51                        14.9
8-day interval              0.51                        14.3
    Significance             NS                          NS
Timing
morning                     0.53                        14.0
evening                     0.50                        15.1
    Significance             NS                          NS         


Table 49. Main effects of frequency and timing of irrigation on mean 
daily hours of leaf wetness and the number and percentage of heads 
with rot on 8 Oct, 1993, NWREC                                       
Treatment           Rotten headsz    Rotten heads     Leaf wetness
                     Number/plot           %        mean daily hours
Frequency		
2-day interval	         5.7	         11.4             15.1
8-day interval	         2.3	          4.4             13.0
    Significance         **	          **                *
Timing
morn